ENGLISH

Pcr Cloning Protocols: From Molecular Cloning to Genetic Engineering

By Bruce A. White

Book information

Publisher
Humana Press
Year
1997
ISBN
9780896034839, 0896034836
Open Library ID
OL8253608M
Language
english
Format
PDF
Filesize
31 MB (32186534 bytes)
Series
Methods in Molecular Biology 067
Pages
472\472
Topic
Technique
Library
mexmat
Time added
2009-07-20 03:45:11

Description

Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.

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