ENGLISH

Homing Endonucleases: Methods and Protocols

By David R. Edgell (eds.)

Book information

Publisher
Humana Press
Year
2014
ISBN
978-1-62703-967-3, 978-1-62703-968-0
DOI
10.1007/978-1-62703-968-0
Language
english
Format
PDF
Filesize
6 MB (6616060 bytes)
Series
Methods in Molecular Biology 1123
Edition
1
Pages
284\288
Topic
Biology Molecular
Time added
2014-04-13 05:00:00
Human Genetics; Genetic Engineering

Description

Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.

Similar books