ENGLISH

Stem Cells and Aging. Methods and Protocols

By Kursad Turksen (ed.)

Book information

Publisher
Humana Press
Year
2019
ISBN
978-1-4939-9712-1, 978-1-4939-9713-8
Language
english
Format
PDF
Filesize
12 MB (12941971 bytes)
Series
Methods in Molecular Biology 2045
Edition
2
Pages
354\353
Topic
Biology Molecular
Time added
2019-10-19 22:09:41

Description

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study. Front Matter ....Pages i-xii Assessing Muscle Stem Cell Clonal Complexity During Aging (Matthew T. Tierney, Michael J. Stec, Alessandra Sacco)....Pages 1-11 Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies (Eusebio Perdiguero, Victoria Moiseeva, Pura Muñoz-Cánoves)....Pages 13-23 Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle (Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler et al.)....Pages 25-36 Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue (Meenakshi Gaur, Marek Dobke, Victoria V. Lunyak)....Pages 37-92 Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures (Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, Francis Rodier)....Pages 93-105 Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells (Courtney R. Ogando, Gilda A. Barabino, Yueh-Hsun Kevin Yang)....Pages 107-117 Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis (Klemen Čamernik, Janja Marc, Janja Zupan)....Pages 119-129 Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells (Klemen Čamernik, Janja Zupan)....Pages 131-144 Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis (Janja Zupan)....Pages 145-154 3D-Embedded Cell Cultures to Study Tendon Biology (Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer et al.)....Pages 155-165 Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis (Brooke Snetsinger, Christina K. Ferrone, Michael J. Rauh)....Pages 167-180 Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants (Sherin Saheera, Renuka R. Nair)....Pages 181-186 Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice (Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, Noël Ghanem)....Pages 187-199 Infrared Spectroscopy and Imaging in Stem Cells and Aging Research (Ceren Aksoy, Feride Severcan)....Pages 201-215 Use of U-STELA for Accurate Measurement of Extremely Short Telomeres (Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis)....Pages 217-224 Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry (Klemen Čamernik, Janja Zupan)....Pages 225-233 Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry (Rasmus Jentoft Boutrup)....Pages 235-244 Methods for Detection of Autophagy in Mammalian Cells (Bindu Singh, Sangeeta Bhaskar)....Pages 245-258 Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells (Yohei Hayashi, Yasuhisa Matsui)....Pages 259-269 Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer (Dan-Ya Wu, Xia Zhang, Yi-Liang Miao)....Pages 271-281 Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD) (Harshini Surendran, Reena J. Rathod, Rajarshi Pal)....Pages 283-298 Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury (Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko et al.)....Pages 299-310 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol (Yuhui Lin)....Pages 311-321 Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments (Shuh-Wen Aung, Noor Hayaty Abu Kasim, Thamil Selvee Ramasamy)....Pages 323-335 CRISPR Base Editing in Induced Pluripotent Stem Cells (Ya-Ju Chang, Christine L. Xu, Xuan Cui, Alexander G. Bassuk, Vinit B. Mahajan, Yi-Ting Tsai et al.)....Pages 337-346 Back Matter ....Pages 347-354

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