ENGLISH

Gene silencing by RNA interference : technology and application

By Muhammad Sohail

Book information

Publisher
CRC Press
Year
2005
ISBN
0849321417, 9780849321412
Language
english
Format
PDF
Filesize
13 MB (14112060 bytes)
Pages
375\368
Time added
2013-09-18 04:00:00

Description

Content: Gene silencing by RNA interference and the role of small interfering RNAs -- Basics of siRNA design and chemical synthesis -- Oligonucleotide scanning arrays in the design of small interfering RNAs -- siRNA production by in vitro transcription -- Production of siRNAs with the application of deoxyribozymes -- Production of siRNA in vitro by enzymatic digestion of double-stranded RNA -- Plasmid-mediated intracellular expression of siRNAs -- Lentiviral vector-mediated delivery of si/shRNA -- Exogenous siRNA delivery: protocols for optimizing delivery to cells -- RNAi in drosophila cell cultures -- RNAi in caenorhabditis elegans -- Delivery of RNAi reagents in C. elegans by microinjection -- RNAi in drosophila embryos -- RNAi in xenopus laevis oocytes -- RNAi in xenopus embryos -- An RNAi-based genomic library for forward genetics in the African trypanosome -- RNAi in the malaria parasite plasmodium -- Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches -- RNAi in transgenic animal models -- RNA interference in mouse models -- Virus-induced RNA silencing in plants -- RNA silencing in plants: biolistic delivery of RNAi reagents -- Agro-infiltration: a versatile tool for RNAi studies in plants.

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