Acute myeloid leukemia : methods and protocols

Description

This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML. Front Matter ....Pages i-xi Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia (Rebecca L. King, Adam Bagg)....Pages 1-17 Cytochemical Staining (Michele E. Paessler, Marybeth Helfrich, Gerald B. W. Wertheim)....Pages 19-32 Immunohistochemistry in Acute Myeloid Leukemia (Michael W. Cruise)....Pages 33-49 Immunophenotyping by Multiparameter Flow Cytometry (Weina Chen, Hung S. Luu)....Pages 51-73 Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells (Zhihong Zeng, Marina Konopleva, Michael Andreeff)....Pages 75-86 Design and Application of Multiplex PCR Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies (Naomi Park, George Vassiliou)....Pages 87-99 AMLprofiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia (Marco Alessandrini, Sharon S. Kappala, Michael S. Pepper)....Pages 101-123 Microsphere-Based Assessment of DNA Methylation for AML Prognosis (Gerald B. W. Wertheim, Marlise R. Luskin, Martin Carroll, Stephen R. Master)....Pages 125-136 Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS (Mira Jeong, Anna G. Guzman, Margaret A. Goodell)....Pages 137-149 Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts (Jonathan Schumacher, Philippe Szankasi, Todd W. Kelley)....Pages 151-161 Targeted Next-Generation Sequencing of Acute Leukemia (Eric Konnick, Christina M. Lockwood, David Wu)....Pages 163-184 Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples (Sean R. Landman, Tae Hyun Hwang)....Pages 185-192 A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation (Alvin C. H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, Anskar Y. H. Leung)....Pages 193-218 Measurement of Oncometabolites d-2-Hydroxyglutaric Acid and l-2-Hydroxyglutaric Acid (Patricia M. Jones, Richard Boriack, Eduard A. Struys, Dinesh Rakheja)....Pages 219-234 Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukemia (Daniel H. Wiseman, Tim C. P. Somervaille)....Pages 235-255 Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer (Chang-Sook Hong, Sonja Funk, Theresa L. Whiteside)....Pages 257-265 Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia (Saad S. Kenderian, Carl H. June, Saar Gill)....Pages 267-276 Back Matter ....Pages 277-278