ENGLISH

Lipoproteins and Cardiovascular Disease: Methods and Protocols

By Lita A. Freeman (auth.) , Lita A. Freeman (eds.)

Book information

Publisher
Humana Press
Year
2013
ISBN
978-1-60327-368-8, 978-1-60327-369-5
DOI
10.1007/978-1-60327-369-5
Language
english
Format
PDF
Filesize
4 MB (4498180 bytes)
Series
Methods in Molecular Biology 1027
Edition
1
Pages
391\385
Orientation
yes
Scanned
yes
Time added
2013-08-10 19:23:05
Protein Science; Cardiology

Description

The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.

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