ENGLISH

Epithelial Cell Culture Protocols

By Clare Wise

Book information

Publisher
Humana Press
Year
2010
ISBN
9781617372414, 1617372412
Language
english
Format
PDF
Filesize
7 MB (7008475 bytes)
Series
Methods in Molecular Biology
Edition
1st Edition.
Pages
388\388
Topic
Biology
Library
mexmat
Time added
2009-07-20 03:45:11

Description

Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

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