ENGLISH

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis

By Marie A. Di Berardino (auth.) , Paul J. Verma , Alan O. Trounson (eds.)

Book information

Publisher
Humana Press
Year
2006
ISBN
9781588292803, 1-58829-280-0
DOI
10.1007/978-1-59745-154-3
Open Library ID
OL17199566M
Language
english
Format
PDF
Filesize
7 MB (6981135 bytes)
Series
Methods in Molecular Biology 348
Edition
1
Pages
338\764
Time added
2010-04-25 21:59:20
Cell Biology

Description

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.

Similar books