Bacteriology Methods for the Study of Infectious Diseases

Description

Bacteriology Methods for the Study of Infectious Diseases provides knowledge, understanding and experience of contemporary, robust methodologies for studies into the pathogenicity and virulence of human/animal bacterial pathogens. This book presents contemporary, yet widely utilized methodologies, for the study of pathogenicity and virulence in bacterial pathogens of human and/or animal origin. Protocols are clearly outlined, with lists of required equipment and reagents, alongside underpinning theory. This text will provide undergraduate and postgraduate students with practical guidance for dissertation projects with protocols for individual project ideas that can be developed further, hence a starting point for additional literature searches is also provided. Helps users research dissertations and interdisciplinary research projects Presents a valuable resource that enables researchers from diverse backgrounds to undertake research within the field of infectious diseases Summarizes protocols that give a fundamental start to research, but are highly adaptable or can be built upon and integrated into other methodologiesProvides knowledge, understanding and experience of contemporary, robust methodologies for studies into the pathogenicity and virulence of human/animal bacterial pathogens Cover BACTERIOLOGY METHODS FOR THE STUDY OF INFECTIOUS DISEASES Copyright Acknowledgement One - Fundamental skills for infectious disease research 1.1 Introduction 1.1.1 Health and safety 1.1.2 Basic laboratory preparation, kit and consumables 1.1.3 Pipettes 1.2 Aseptic technique 1.3 Common laboratory units 1.4 Top tips for working safely in the lab 1.5 Notes page Two - Bacterial growth in solid and liquid media 2.1 Introduction 2.2 Media, culture conditions and nutritional requirements 2.2.1 Isolation 2.3 Differential and selective media 2.4 Estimating cell number 2.5 Direct enumeration (viable cells) 2.6 Notes page Three - Microscopy and staining 3.1 Introduction 3.2 Light microscopy 3.3 Preparing slides 3.4 Stains 3.5 Using the light microscope 3.6 Other types of microscopy 3.7 Notes page Four - Antimicrobial testing 4.1 Introduction 4.1.1 Antibiotics and targets 4.1.2 Disc diffusion 4.1.3 Antibiotic strip tests 4.1.4 Broth dilution 4.1.5 Synergy 4.1.6 Novel compound testing 4.2 Notes page Five - Cell culture-based infection models 5.1 Introduction 5.1.1 Which species of bacteria are you working with and which is the best cell line to use? 5.1.2 Cell culture and enumeration 5.1.3 Viability and multiplicity of infection 5.1.4 Bacterial attachment and internalisation/invasion 5.1.5 Using cell culture-based infection models to support previous observations and plan the next experiments 5.2 Notes page Six - Biofilm models to understand infectious diseases 6.1 Introduction 6.1.1 Does the bacterium form a biofilm? 6.1.2 How many bacteria are in the biofilm and are they viable? 6.1.3 Applications of simple static biofilm models 6.1.4 Other types of static biofilm model 6.1.5 Studying biofilms under conditions of flow 6.2 Notes page Seven - Gene expression analysis 7.1 Introduction 7.2 Sample preparation and DNA extraction 7.3 Primers 7.4 Electrophoresis to analyse polymerase chain reaction products 7.5 Quantitative polymerase chain reaction 7.6 Troubleshooting 7.7 Notes page Eight - Screening for common virulence traits 8.1 Introduction 8.1.1 Agar-based tests 8.1.2 Non-agar plate-based assays 8.1.3 Virulence genes 8.2 Notes page Nine- Community composition studies 9.1 Introduction 9.1.1 Using culture methods to study microbial communities 9.1.2 Using culture methods to assess rates of mutation within a microbial community 9.1.3 Analysis of microbial communities using molecular techniques 9.1.4 Considering the impact of environment 9.1.5 Summary 9.2 Notes page Ten - Invertebrate infection models 10.1 Introduction 10.1.1 Caenorhabditis elegans 10.1.2 Galleria mellonella 10.1.2.1 Experimental planning 10.1.2.2 Experimental protocol 10.1.2.3 Testing an antimicrobial treatment 10.1.3 Kaplan–Meier analysis of data 10.1.4 Example of information to be entered into spreadsheet to calculate Kaplan–Meier survival probability based on G. mellonell... 10.2 Summary 10.3 Notes page References Webpages Index A B C D E F G H I K L M N O P Q R S T U V W Z Back Cover