ENGLISH

Electron Microscopy Methods and Protocols

By Heather A. Davies (auth.) , M. A. Nasser Hajibagheri (eds.)

Book information

Publisher
Humana Press
Year
1999
DOI
10.1385/1592592015
Language
english
Format
PDF
Filesize
4 MB (4085406 bytes)
Series
Methods in Molecular Biology 117
Pages
277\277
Topic
Biology
Library
mexmat
Time added
2009-07-20 03:45:11
Biochemistrygeneral

Description

Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.

Similar books