Prions : Methods and Protocols

Description

This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field. Read more... Abstract: This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Read more... Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Purification and Fibrillation of Full-Length Recombinant PrP (Natallia Makarava, Regina Savtchenko, Ilia V. Baskakov)....Pages 3-22 Method for Folding of Recombinant Prion Protein to Soluble β-Sheet Secondary Structure (Laura J. Ellett)....Pages 23-26 Analysis of Prion Protein Conformation Using Circular Dichroism Spectroscopy (Laura J. Ellett, Vanessa A. Johanssen)....Pages 27-34 Analysis of Prion Protein Structure Using Nuclear Magnetic Resonance Spectroscopy (Ivana Biljan, Gregor Ilc, Janez Plavec)....Pages 35-49 Immunodetection of PrPSc Using Western Immunoblotting Techniques (Gerald S. Baron, Gregory J. Raymond)....Pages 51-66 Analysis of miRNA Signatures in Neurodegenerative Prion Disease (Shayne A. Bellingham, Andrew F. Hill)....Pages 67-80 Front Matter ....Pages 81-81 Cell Biology Approaches to Studying Prion Diseases (Suzette A. Priola)....Pages 83-94 Expression of Heterologous PrP and Prion Propagation in RK13 Cells (Zaira E. Arellano-Anaya, Alvina Huor, Pascal Leblanc, Olivier Andréoletti, Didier Vilette)....Pages 95-104 Generation of Infectious Prions and Detection with the Prion-Infected Cell Assay (Laura J. Vella, Bradley Coleman, Andrew F. Hill)....Pages 105-118 Analysis of Cellular Prion Protein Endoproteolytic Processing (Victoria Lewis)....Pages 119-132 Cellular Analysis of Adult Neural Stem Cells for Investigating Prion Biology (Cathryn L. Haigh)....Pages 133-145 Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons (Giuseppe D. Ciccotosto, Metta Jana, Roberto Cappai)....Pages 147-165 Front Matter ....Pages 167-167 Methods of Protein Misfolding Cyclic Amplification (Natallia Makarava, Regina Savtchenko, Ilia V. Baskakov)....Pages 169-183 RT-QuIC Assays for Prion Disease Detection and Diagnostics (Christina D. Orrù, Bradley R. Groveman, Andrew G. Hughson, Matteo Manca, Lynne D. Raymond, Gregory J. Raymond et al.)....Pages 185-203 A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein (Natalia Fernández-Borges, Hasier Eraña, Saioa R. Elezgarai, Chafik Harrathi, Vanesa Venegas, Joaquín Castilla)....Pages 205-216 Front Matter ....Pages 217-217 Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases (Julie A. Moreno, Glenn C. Telling)....Pages 219-252 In Vivo-Near Infrared Imaging of Neurodegeneration (Victoria A. Lawson, Carolin Tumpach, Cathryn L. Haigh, Simon C. Drew)....Pages 253-262 Strain Typing of Prion Diseases Using In Vivo Mouse Models (Aileen Boyle, Kris Hogan, Jean C. Manson, Abigail B. Diack)....Pages 263-283 Preparation and Immunostaining of the Myenteric Plexus of Prion-Infected Mice (Laura J. Ellett, Victoria A. Lawson)....Pages 285-292 Front Matter ....Pages 293-293 Cell Culture Methods for Screening of Prion Therapeutics (Hilary E. McMahon)....Pages 295-304 Real-Time Quaking-Induced Conversion for Diagnosis of Prion Disease (Katsuya Satoh, Ryuichiro Atarashi, Noriyuki Nishida)....Pages 305-310 Methods for Molecular Diagnosis of Human Prion Disease (Jonathan D. F. Wadsworth, Gary Adamson, Susan Joiner, Lara Brock, Caroline Powell, Jacqueline M. Linehan et al.)....Pages 311-346 Molecular Subtyping of PrPres in Human Sporadic CJD Brain Tissue (G. M. Klug, V. Lewis, S. J. Collins)....Pages 347-354 Front Matter ....Pages 355-355 Intercellular Prion-Like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture (Leslie I. Grad, Edward Pokrishevsky, Neil R. Cashman)....Pages 357-367 Back Matter ....Pages 369-371