Biophysics of RNA-Protein Interactions: A Mechanistic View

Description

RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling. Front Matter ....Pages i-vii Front Matter ....Pages 1-1 How Proteins Recognize RNA (Rajan Lamichhane)....Pages 3-21 The Interaction Between L7Ae Family of Proteins and RNA Kink Turns (Lin Huang, David M. J. Lilley)....Pages 23-37 Evolving Methods in Defining the Role of RNA in RNP Assembly (Jaya Sarkar, Jong Chan Lee, Sua Myong)....Pages 39-55 Single-Molecule Studies of Exonucleases: Following Cleavage Actions One Step at a Time (Gwangrog Lee)....Pages 57-84 Fitting in the Age of Single-Molecule Experiments: A Guide to Maximum-Likelihood Estimation and Its Advantages (Behrouz Eslami-Mosallam, Iason Katechis, Martin Depken)....Pages 85-105 Front Matter ....Pages 107-107 A Single-Molecule View on Cellular and Viral RNA Synthesis (Eugen Ostrofet, Flavia Stal Papini, Anssi M. Malinen, David Dulin)....Pages 109-141 Single-Molecule Optical Tweezers Studies of Translation (Xiaohui Qu)....Pages 143-164 Front Matter ....Pages 165-165 Biophysical and Biochemical Approaches in the Analysis of Argonaute–MicroRNA Complexes (Sujin Kim, Yoosik Kim)....Pages 167-188 Biophysics of RNA-Guided CRISPR Immunity (Luuk Loeff, Chirlmin Joo)....Pages 189-210 Dynamics of MicroRNA Biogenesis (Mohamed Fareh)....Pages 211-249