ENGLISH

Cancer Metabolism: Methods and Protocols

By Majda Haznadar

Book information

Publisher
Humana Press
Year
2019
ISBN
1493990268, 9781493990269
Language
english
Format
PDF
Filesize
13 MB (14054508 bytes)
Edition
Hardcover
Pages
495\491
Time added
2019-03-28 10:16:05

Description

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,Cancer Metabolism: Methods and Protocolsaims to be an essential compilation of protocols utilized in many laboratories. Front Matter ....Pages i-xi Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction (Daniel R. Crooks, Teresa W.-M. Fan, W. Marston Linehan)....Pages 1-27 Imaging Cancer Metabolism with Positron Emission Tomography (PET) (Timothy H. Witney, David Y. Lewis)....Pages 29-44 Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells (Debanti Sengupta, Guillem Pratx)....Pages 45-53 Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation Dynamics in Cancer Cell Metabolism and Epigenetics (Alice C. Newman, Christiaan F. Labuschagne, Karen H. Vousden, Oliver D. K. Maddocks)....Pages 55-67 Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) (Sarah Creed, Matthew McKenzie)....Pages 69-76 Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells (David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, Carmen Chak-Lui Wong)....Pages 77-99 Determination of Polarization of Resident Macrophages and Their Effect on the Tumor Microenvironment (Ioannis S. Pateras, Tomer Cooks)....Pages 101-112 Quantitation of Macropinocytosis in Cancer Cells (Koen M. O. Galenkamp, Basheer Alas, Cosimo Commisso)....Pages 113-123 Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation (Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, Alessandro Carrer)....Pages 125-147 Methods to Measure Autophagy in Cancer Metabolism (Natalia von Muhlinen)....Pages 149-173 Lipidomic Analysis of Cancer Cell and Tumor Tissues (Islam Sk Ramiz, Soumen Kanti Manna)....Pages 175-204 Mass Spectrometry-Based Profiling of Metabolites in Human Biofluids (Tanushree Chakraborty, Soumen Kanti Manna)....Pages 205-234 Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile Through Extraction and Derivatization of Polar Metabolites (Jodi Rattner, Farshad Farshidfar, Oliver F. Bathe)....Pages 235-249 Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy (Beata Mickiewicz, M. Eric Hyndman, Hans J. Vogel)....Pages 251-274 Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging (Shibdas Banerjee, Soumen Kanti Manna)....Pages 275-297 Compositional Analysis of the Human Microbiome in Cancer Research (Elisa Morales, Jun Chen, K. Leigh Greathouse)....Pages 299-335 Assessing Metabolic Dysregulation in Muscle During Cachexia (Myriam Y. Hsu, Paolo E. Porporato, Elisabeth Wyart)....Pages 337-352 Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells (Jing Zhang, Qing Zhang)....Pages 353-363 Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging (Thomas S. Blacker, Michael D. E. Sewell, Gyorgy Szabadkai, Michael R. Duchen)....Pages 365-387 Overview of Characterizing Cancer Glycans with Lectin-Based Analytical Methods (Amanda J. Pearson, Elyssia S. Gallagher)....Pages 389-408 Hyperpolarized MRI for Studying Tumor Metabolism (Mikko I. Kettunen)....Pages 409-426 Overview of Glutamine Dependency and Metabolic Rescue Protocols (Shuo Qie, Dan He, Nianli Sang)....Pages 427-439 Integration of Metabolomics and Transcriptomics to Identify Gene-Metabolite Relationships Specific to Phenotype (Andrew Patt, Jalal Siddiqui, Bofei Zhang, Ewy Mathé)....Pages 441-468 Biclustering Analysis of Co-regulation Patterns in Nuclear-Encoded Mitochondrial Genes and Metabolic Pathways (Robert B. Bentham, Kevin Bryson, Gyorgy Szabadkai)....Pages 469-478 Using the Human Genome-Scale Metabolic Model Recon 2 for Steady-State Flux Analysis of Cancer Cell Metabolism (Lake-Ee Quek, Nigel Turner)....Pages 479-489 Back Matter ....Pages 491-495

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