Chiral Separations: Methods and Protocols

Description

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry. Front Matter ....Pages i-xiv Recognition Mechanisms of Chiral Selectors: An Overview (Gerhard K. E. Scriba)....Pages 1-33 Enantioseparation by Thin-Layer Chromatography (Rituraj Dubey, Ravi Bhushan)....Pages 35-44 Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase (Sheng-Ming Xie, Jun-Hui Zhang, Li-Ming Yuan)....Pages 45-55 Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS (Toshimasa Toyo’oka)....Pages 57-79 Chiral Mobile-Phase Additives in HPLC Enantioseparations (Lushan Yu, Shengjia Wang, Su Zeng)....Pages 81-91 Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High-Performance Liquid Chromatography: An Overview (Bezhan Chankvetadze)....Pages 93-126 HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions (Roberto Cirilli)....Pages 127-146 Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC (Jie Zhou, Jian Tang, Weihua Tang)....Pages 147-157 HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases (Xiaoxuan Li, Yong Wang)....Pages 159-169 Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector (Liang Zhao, Hui Li, Shuqing Dong, Yanping Shi)....Pages 171-181 Cyclofructans as Chiral Selectors: An Overview (Garrett Hellinghausen, Daniel W. Armstrong)....Pages 183-200 High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview (István Ilisz, Tímea Orosz, Antal Péter)....Pages 201-237 Application of Sub-2 Micron Particle Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography (Chiara Fanali, Salvatore Fanali)....Pages 239-250 Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview (István Ilisz, Attila Bajtai, Antal Péter, Wolfgang Lindner)....Pages 251-277 Enantioseparations by High-Performance Liquid Chromatography Based on Chiral Ligand Exchange (Federica Ianni, Lucia Pucciarini, Andrea Carotti, Roccaldo Sardella, Benedetto Natalini)....Pages 279-302 Applications of Chiral Supercritical Fluid Chromatography (Emmanuelle Lipka)....Pages 303-319 Chiral Separations by Countercurrent Chromatography (Sheng-Qiang Tong)....Pages 321-337 Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations (Gerhard K. E. Scriba, Pavel Jáč)....Pages 339-356 Application of Dual-Cyclodextrin Systems in Capillary Electrophoresis Enantioseparations (Anne-Catherine Servais, Marianne Fillet)....Pages 357-364 Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer (Joachim Wahl, Ulrike Holzgrabe)....Pages 365-371 Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins (Anne-Catherine Servais, Marianne Fillet)....Pages 373-381 Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes (Lijuan Wang, Xu Hou, Fan Zhang, Ying Liu, Yimeng Ren, Hongyuan Yan)....Pages 383-389 Chiral Capillary Electrophoresis-Mass Spectrometry (María Castro-Puyana, María Luisa Marina)....Pages 391-405 Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography (Wan Aini Wan Ibrahim, Siti Munirah Abd Wahib, Dadan Hermawan, Mohd Marsin Sanagi)....Pages 407-416 Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry (Vijay Patel, Shahab A. Shamsi)....Pages 417-444 Application of an (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography (Wonjae Lee, Kyung Tae Kim, Jong Seong Kang)....Pages 445-452 Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview (Luiz Carlos Klein-Júnior, Debby Mangelings, Yvan Vander Heyden)....Pages 453-478 Back Matter ....Pages 479-487