ENGLISH

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

By Peter G. Isaac (auth.) , Peter G. Isaac (eds.)

Book information

Publisher
Humana Press
Year
1994
ISBN
978-0-89603-254-5, 978-1-59259-515-0
DOI
10.1385/089603254X
Language
english
Format
PDF
Filesize
23 MB (24301362 bytes)
Series
Methods in Molecular Biology™ 28
Pages
263\263
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Cell Biology

Description

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

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