ENGLISH

Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques: Kidney, Lung, Heart, Intestine and Mesenteric Lymph Node

By George Parker

Book information

Publisher
Academic Press
Year
2022
ISBN
0323913938, 9780323913935
Language
english
Format
PDF
Filesize
26 MB (27309044 bytes)
Pages
292\294
Topic
Medicine Histology
Time added
2022-10-04 00:56:54

Description

Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques: Kidney, Lung, Heart, Intestine and Mesenteric Lymph Node provides a thoroughly illustrated review of the tissue damage and reparative changes associated with standardized irradiation doses in rhesus macaques. In addition to time-sequenced, routinely stained histologic sections, the book presents results of numerous histochemical, immunohistochemical and chromogenic in-situ stains that provide insights into the pathogenesis of radiation-associated tissue injury and repair. This book is compiled and written by a board-certified veterinary pathologist with more than 40 years of experience in the interpretation of experimentally induced tissue alterations in laboratory animals. This is an important resource for researchers in the field of animal science radiation injury, including radiation oncologists and individuals involved in disaster preparedness related to accidental or deliberate radiation exposure in large populations. Front Cover Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques Copyright Page Contents Foreword Preface Overview of studies Irradiation protocol Animal cage-side observations Medical management Dexamethasone treatment, nonsedated respiratory rate Euthanasia Necropsy and histology procedures Acknowledgments Funding References Further reading 1 Radiation-associated changes in the kidney of rhesus macaques Overview Staining and figures list Detailed microscopic observations Hyaline casts Glomerular congestion Glomerular thrombosis Segmental glomerular capillary dilatation Increased mesangial matrix Inflammatory cell infiltration Miscellaneous changes observed in H&E-stained sections Interstitial fibrosis—trichrome stain Glomerular fibrosis—trichrome stain PAS+ mesangial deposits Phosphotungstic acid-hematoxylin stain Perls’ iron stain Toluidine blue stain Tryptase immunohistochemical stain Alpha smooth muscle actin immunohistochemical stain von Willebrand factor immunohistochemical stain CD31 immunohistochemical stain Collagen 1 immunohistochemical stain Connective tissue growth factor immunohistochemical stain Transforming growth factor β immunohistochemical stain IL-22 immunohistochemical stain IL-22 receptor (IL-22R) immunohistochemical stain Pro-renin receptor immunohistochemical stain CD38 immunohistochemical stain CD68 immunohistochemical stain CD20 immunohistochemical stain CD3 immunohistochemical stain Myeloperoxidase immunohistochemical stain CD163 immunohistochemical stain Tryptase immunohistochemical stain References 2 Radiation-associated changes in the lung of rhesus macaques Overview Staining and figures list Detailed microscopic observations Alveolar macrophage accumulation Alveolar edema Type 2 pneumocyte hyperplasia Hyperplasia, alveolar–bronchiolar Lymphocyte infiltration Neutrophil infiltration Emphysema Myxoid cells Interstitial and pleural fibrosis Toluidine blue stain Alpha smooth muscle actin immunohistochemical stain Tryptase immunohistochemical stain CD31 immunohistochemical stain Myeloperoxidase immunohistochemical stain CD163 immunohistochemical stain CD206 immunohistochemical stain CD38 immunohistochemical stain Collagen 1 immunohistochemical stain Transforming growth factor β immunohistochemical stain Connective tissue growth factor immunohistochemical stain References 3 Radiation-associated changes in the heart of rhesus macaques Overview Stains and related figures Detailed microscopic observations Toluidine blue stain Tryptase immunohistochemical stain Alpha smooth muscle actin immunohistochemical stain Galectin-3 immunohistochemical staining Caspase 3 immunohistochemical staining References 4 Radiation-associated changes in the jejunum of rhesus macaques Overview Staining and figures list Detailed microscopic observations Mucosal epithelial depletion Trichrome stain Periodic acid/Schiff (PAS)/Alcian blue stain Leukocyte infiltration Toluidine blue stain Tryptase immunohistochemical stain Alpha smooth muscle actin immunohistochemical stain MxA immunohistochemical stain Lipopolysaccharide core antigen immunohistochemical stain Ki-67 immunohistochemical stain Lgr5 in situ hybridization stain Myeloperoxidase immunohistochemical stain CD38 immunohistochemical stain CD163 immunohistochemical stain CD3 immunohistochemical stain CD13 immunohistochemical stain Bmi-1 immunohistochemical stain IL22 immunohistochemical stain IL22R immunohistochemical stain Collagen 1 immunohistochemical stain Transforming growth factor β immunohistochemical stain Connective tissue growth factor immunohistochemical stain 5 Radiation-associated changes in the colon of rhesus macaques Overview Staining and figure lists Detailed colon observations Leukocyte infiltration Trichrome stain PAS/Alcian blue stain Toluidine blue stain Tryptase immunohistochemical stain Alpha smooth muscle actin immunohistochemical stain MxA immunohistochemical stain Lipopolysaccharide core antigen immunohistochemical stain Ki-67 immunohistochemical stain Myeloperoxidase immunohistochemical stain CD3 immunohistochemical stain CD13 immunohistochemical stain Bmi-1 immunohistochemical stain IL-22 immunohistochemical stain IL-22R immunohistochemical stain Collagen 1 immunohistochemical stain Transforming growth factor β immunohistochemical stain Connective tissue growth factor immunohistochemical stain 6 Radiation-associated changes in the mesenteric lymph node of rhesus macaques Overview Staining and figures list Detailed microscopic observations Routine histologic stain Trichrome stain Toluidine blue stain Tryptase immunohistochemical stain Alpha-smooth muscle actin immunohistochemical stain MxA immunohistochemical stain LPS core immunohistochemical stain Myeloperoxidase immunohistochemical stain Collagen 1 immunohistochemical stain Connective tissue growth factor immunohistochemical stain Transforming growth factor β immunohistochemical stain Index Back Cover

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