ENGLISH

Plant MicroRNAs: Methods and Protocols

By Stefan de Folter

Book information

Publisher
Springer New York;Humana Press
Year
2019
ISBN
978-1-4939-9041-2, 978-1-4939-9042-9
Language
english
Format
PDF
Filesize
10 MB (10437956 bytes)
Series
Methods in Molecular Biology 1932
Edition
1st ed.
Pages
XII, 363\357
Time added
2019-04-19 19:00:00
Life Sciences; Plant Genetics and Genomics; Gene E

Description

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science. Front Matter ....Pages i-xii An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants (Alma Armenta-Medina, C. Stewart Gillmor)....Pages 1-14 The Plant microRNA Pathway: The Production and Action Stages (Joseph L. Pegler, Christopher P. L. Grof, Andrew L. Eamens)....Pages 15-39 Plant miRNA Conservation and Evolution (Lydia Gramzow, Günter Theißen)....Pages 41-50 Bioinformatic Analysis of Small RNA Sequencing Libraries (Ricardo A. Chávez Montes, Fabiola Jaimes-Miranda, Stefan de Folter)....Pages 51-63 An Overview of miRNA and miRNA Target Analysis Tools (Anna Lukasik, Piotr Zielenkiewicz)....Pages 65-87 miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences (Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, Chuang Ma)....Pages 89-97 Prediction of Plant miRNA Targets (Priyanka Pandey, Prashant K. Srivastava, Shree P. Pandey)....Pages 99-107 Isolation and Detection Methods of Plant miRNAs (Pedro Fernando Vera-Hernández, Stefan de Folter, Flor de Fátima Rosas-Cárdenas)....Pages 109-120 Northern Blot Analysis of microRNAs and Other Small RNAs in Plants (Carlos De la Rosa, José Luis Reyes)....Pages 121-129 miRNA Detection by Stem-Loop RT-qPCR in Studying microRNA Biogenesis and microRNA Responsiveness to Abiotic Stresses (Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak et al.)....Pages 131-150 Detection of miRNAs by Tissue Printing and Dot Blot Hybridization (Marcelino Martínez Núñez, Stefan de Folter, Flor de Fátima Rosas-Cárdenas)....Pages 151-157 In Situ Localization of Small RNAs in Plants (Cristina F. Marco, Damianos S. Skopelitis, Marja C. P. Timmermans)....Pages 159-173 Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs (Andrea Tovar-Aguilar, Karla A. Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I. González-Jaime, Genaro Patiño-López, Vadim Perez-Koldenkova et al.)....Pages 175-185 A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy (Andrea Gómez-Felipe, Stefan de Folter)....Pages 187-195 Degradome Sequencing in Plants (Shih-Shun Lin, Yihua Chen, Mei-Yeh Jade Lu)....Pages 197-213 Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System (Damien Formey, José Ángel Martín-Rodríguez, Georgina Hernández)....Pages 215-226 The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis (Gigi Wong, Anthony A. Millar)....Pages 227-238 Mimicry Technology: A Versatile Tool for Small RNA Suppression (Luis Manuel Villar-Martin, Ignacio Rubio-Somoza)....Pages 239-245 Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants (Alberto Carbonell)....Pages 247-260 Detection of MicroRNA Processing Intermediates Through RNA Ligation Approaches (Belén Moro, Arantxa M. L. Rojas, Javier F. Palatnik)....Pages 261-283 Analysis of Methylation Status of Plant MicroRNAs (Susu Chen, Guodong Ren)....Pages 285-293 Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 (Laura Arribas-Hernández, Maria Louisa Vigh, Peter Brodersen)....Pages 295-316 The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs (Shengben Li)....Pages 317-333 Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae (Gloria León-Martínez, Edgar Demesa-Arévalo, Jean-Philippe Vielle-Calzada)....Pages 335-345 Detection of Histone Modifications Associated with miRNAs (Sara Hernández-Castellano, Clelia De-la-Peña)....Pages 347-357 Back Matter ....Pages 359-363

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