Advanced Techniques in Diagnostic Microbiology: Volume 1: Techniques

Description

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions. Front Matter ....Pages i-xiv Automated Blood Cultures (Xiang Y. Han)....Pages 1-13 Laboratory Automation in Clinical Bacteriology (Antony Croxatto)....Pages 15-32 Biochemical Profile-Based Microbial Identification Systems (Safina Hafeez, Jaber Aslanzadeh)....Pages 33-67 Advanced Phenotypic Antimicrobial Susceptibility Testing Methods (Charles W. Stratton)....Pages 69-98 Rapid Microbial Antigen Tests (Sheldon Campbell, Marie L. Landry)....Pages 99-125 Antibody Detection: Principles and Applications (Yun F. (Wayne) Wang)....Pages 127-147 Procalcitonin and Other Host-Response-Based Biomarkers for Evaluation of Infection and Guidance of Antimicrobial Treatment (Philipp Schuetz, Ramon Sager, Yannick Wirz, Beat Mueller)....Pages 149-160 Functional Assessment of Microbial, Viral, and Parasitic Infections Using Real-Time Cellular Analysis (Dazhi Jin, Xiao Xu, Min Zheng, Alex Mira, Brandon J. Lamarche, Alex B. Ryder)....Pages 161-198 Cellular Fatty Acid-Based Microbial Identification and Antimicrobial Susceptibility Testing (Nicole Parrish, Stefan Riedel)....Pages 199-209 MALDI-TOF Mass Spectrometry-Based Microbial Identification and Beyond (Alexander Mellmann, Johannes Müthing)....Pages 211-234 Transcriptomic Techniques in Diagnostic Microbiology (Zachary E. Holcomb, Ephraim L. Tsalik)....Pages 235-259 The Use of Microbial Metabolites for the Diagnosis of Infectious Diseases (Mahesh J. Thalavitiya Acharige, Seena S. Koshy, Sophia Koo)....Pages 261-272 Nucleic Acid Extraction and Enrichment (Jeong Hwan Shin)....Pages 273-292 Nonamplified Probe-Based Microbial Detection and Identification (Fann Wu, Tao Hong, Phyllis Della-Latta)....Pages 293-304 Molecular Typing Techniques: State of the Art (Richard V. Goering)....Pages 305-326 PCR and Its Variations (Eleanor A. Powell, Michael Loeffelholz)....Pages 327-346 Non-PCR Amplification Techniques (Rosemary C. She, Ted E. Schutzbank, Elizabeth M. Marlowe)....Pages 347-375 Real-Time and Digital PCR for Nucleic Acid Quantification (Alexander J. McAdam)....Pages 377-387 Direct Nucleotide Sequencing for Amplification Product Identification (Tao Hong)....Pages 389-402 Solid and Suspension Microarrays for Detection and Identification of Infectious Diseases (Sherry Dunbar, Janet Farhang, Shubhagata Das, Sabrina Ali, Heng Qian)....Pages 403-449 Real-Time Detection of Amplification Products Through Fluorescence Quenching or Energy Transfer (Caitlin Otto, Shihai Huang)....Pages 451-480 PCR/Electrospray Ionization-Mass Spectrometry as an Infectious Disease Diagnostic Tool (Volkan Özenci, Kristoffer Strålin)....Pages 481-490 Nucleic Acid Amplicons Detected and Identified by T2 Magnetic Resonance (Jessica L. Snyder, Heather S. Lapp, Zhi-Xiang Luo, Brendan Manning, Thomas J. Lowery)....Pages 491-503 Molecular Contamination and Amplification Product Inactivation (Susan Sefers, Jonathan E. Schmitz)....Pages 505-526 Back Matter ....Pages 527-541