Experimental Cholestasis Research

Description

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology. Front Matter ....Pages i-xiii Mechanisms of Drug-Induced Cholestasis (Eva Gijbels, Mathieu Vinken)....Pages 1-14 LC-MS/MS Analysis of Bile Acids in In Vitro Samples (Debbie Dewaele, Pieter Annaert, Eef Hoeben)....Pages 15-23 Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis (Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, Jan G. Hengstler)....Pages 25-53 Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function (Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, Pieter Annaert)....Pages 55-73 The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity (Jonathan P. Jackson, Kenneth R. Brouwer)....Pages 75-85 In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids (Stef De Lombaerde, Sara Neyt, Christian Vanhove, Filip De Vos)....Pages 87-98 Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy (Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M. P. Rodrigues, Manuel Prieto, Fábio Fernandes)....Pages 99-115 The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis (Reza Heidari, Hossein Niknahad)....Pages 117-132 Measuring Apoptosis and Necrosis in Cholestatic Liver Injury (Benjamin L. Woolbright, Hartmut Jaeschke)....Pages 133-147 Necrosome Formation and Necroptosis in Experimental Cholestasis (Marta B. Afonso, Cecília M. P. Rodrigues)....Pages 149-162 Proteomics in Primary Biliary Cholangitis (You Li, Ruqui Tang, Patrick S. C. Leung, M. Eric Gershwin, Xiong Ma)....Pages 163-173 Transcriptomic Analysis of Cholestatic Compounds In Vitro (Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, Lysiane Richert)....Pages 175-186 System Microscopy of Stress Response Pathways in Cholestasis Research (Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, Bob van de Water)....Pages 187-202 Mouse Models for Diseases in the Cholangiocyte Lineage (Noémi Van Hul, Urban Lendahl, Emma R. Andersson)....Pages 203-236 Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis (Sanne Van Campenhout, Hans Van Vlierberghe, Lindsey Devisscher)....Pages 237-247 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research (Elisa Pose, Pau Sancho-Bru, Mar Coll)....Pages 249-257 A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis (Sujit K. Mohanty, Bryan Donnelly, Haley Temple, Gregory M. Tiao)....Pages 259-271 Zebrafish as a Model to Study Cholestatic Liver Diseases (Duc-Hung Pham, Chunyue Yin)....Pages 273-289 Setup and Use of HepaRG Cells in Cholestasis Research (Christiane Guguen-Guillouzo, André Guillouzo)....Pages 291-312 Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis (Sreenivasa C. Ramaiahgari, Stephen S. Ferguson)....Pages 313-323 Establishment of Sandwich Cultures of Primary Human Hepatocytes (Eva Gijbels, Tamara Vanhaecke, Mathieu Vinken)....Pages 325-333 Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes (Neel Deferm, Lysiane Richert, Pieter Van Brantegem, Tom De Vocht, Bing Qi, Peter de Witte et al.)....Pages 335-350 Ex Vivo Model in Cholestasis Research (Ruby E. H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, Peter Olinga)....Pages 351-362 Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies (Carol J. Soroka, David N. Assis, James L. Boyer)....Pages 363-372 Use of Biliary Organoids in Cholestasis Research (Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, Ludovic Vallier)....Pages 373-382 In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis (Sankalp Jain, Gerhard F. Ecker)....Pages 383-396 Back Matter ....Pages 397-399