ENGLISH

G Protein-Coupled Receptor Signaling: Methods and Protocols

By Mario Tiberi

Book information

Publisher
Springer New York; Humana
Year
2019
ISBN
978-1-4939-9120-4;978-1-4939-9121-1
Language
english
Format
PDF
Filesize
11 MB (11021297 bytes)
Series
Methods in Molecular Biology 1947
Edition
1st ed.
Pages
XV, 406\401
Time added
2019-09-18 12:16:59
Life Sciences; Protein Science; Pharmacology/Toxic

Description

This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com. Front Matter ....Pages i-xv Front Matter ....Pages 1-1 Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics (Chenyi Liao, Victor May, Jianing Li)....Pages 3-19 Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs (Katsufumi Tomobe, Eiji Yamamoto, Kenji Yasuoka)....Pages 21-30 Expression and Purification of a Functional E. coli13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies (Fabian Bumbak, Ross A. D. Bathgate, Daniel J. Scott, Paul R. Gooley)....Pages 31-55 A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties (Anne Zemella, Theresa Richter, Lena Thoring, Stefan Kubick)....Pages 57-77 Front Matter ....Pages 79-79 Furan Cross-Linking Technology for Investigating GPCR–Ligand Interactions (Marleen Van Troys, Willem Vannecke, Christophe Ampe, Annemieke Madder)....Pages 81-102 Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely Tethered Ligands (Amanda Acosta-Ruiz, Johannes Broichhagen, Joshua Levitz)....Pages 103-136 Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assays on GPCRs (Sridévi M. Ramanoudjame, Lucie Esteoulle, Stéphanie Riché, Jean-François Margathe, Thierry Durroux, Iuliia A. Karpenko et al.)....Pages 137-147 Front Matter ....Pages 149-149 Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-oligomer Pharmacology (Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin et al.)....Pages 151-168 Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches (Kyla Bourque, Dominic Devost, Asuka Inoue, Terence E. Hébert)....Pages 169-182 Measuring GPCR Stoichiometry Using Types-1, -2, and -3 Bioluminescence Resonance Energy Transfer-Based Assays (James H. Felce, John R. James, Simon J. Davis)....Pages 183-197 Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein–Protein Interactions (Amina M. Bagher, Melanie E. M. Kelly, Eileen M. Denovan-Wright)....Pages 199-215 Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters (Andreas Koschinski, Manuela Zaccolo)....Pages 217-237 Front Matter ....Pages 239-239 Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays (Geneviève Hamel-Côté, Fanny Lapointe, Jana Stankova)....Pages 241-256 Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay (Geneviève Laroche, Patrick M. Giguère)....Pages 257-267 Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry (Burcu Hasdemir, Juan A. Oses-Prieto, Alma Burlingame, Aditi Bhargava)....Pages 269-285 Front Matter ....Pages 287-287 Monitoring the Aggregation of GPCRs by Fluorescence Microscopy (Samuel Génier, Jade Degrandmaison, Christine L. Lavoie, Louis Gendron, Jean-Luc Parent)....Pages 289-302 Combining RNAi and Immunofluorescence Approaches to Investigate Post-endocytic Sorting of GPCRs into Multivesicular Bodies (Xuezhi Li, Stéphanie Rosciglione, Andréanne Laniel, Christine Lavoie)....Pages 303-322 Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy (Fabiana A. Caetano Crowley, Bryan Heit, Stephen S. G. Ferguson)....Pages 323-336 Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy (Kim C. Jonas, Aylin C. Hanyaloglu)....Pages 337-348 Front Matter ....Pages 349-349 Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug (Marc López-Cano, Joan Font, Amadeu Llebaria, Víctor Fernández-Dueñas, Francisco Ciruela)....Pages 351-359 Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice (Dmitry Akhmedov, Nicholas S. Kirkby, Jane A. Mitchell, Rebecca Berdeaux)....Pages 361-376 Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents (Xavier Toussay, Mario Tiberi, Baptiste Lacoste)....Pages 377-387 Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs (Martha Hinton, Anurag Singh Sikarwar, Shyamala Dakshinamurti)....Pages 389-401 Back Matter ....Pages 403-406

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