Super-resolution microscopy : methods and protocols

Description

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy:Methods and Protocols is a valuable resource for both established and novel researchers and users in this field. Front Matter ....Pages i-xi Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics (Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle et al.)....Pages 1-13 Managing the Introduction of Super-Resolution Microscopy into a Core Facility (Jeffrey A. Kamykowski, Brian Storrie)....Pages 15-19 Live-Cell STED Imaging with the HyPer2 Biosensor (Natalia M. Mishina, Vsevolod V. Belousov)....Pages 21-28 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope (André Klauss, Carsten Hille)....Pages 29-44 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo (Mirelle J. T. ter Veer, Thomas Pfeiffer, U. Valentin Nägerl)....Pages 45-64 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells (Roman S. Erdmann, Derek Toomre, Alanna Schepartz)....Pages 65-78 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination (Ulrike Engel)....Pages 79-94 Correlative SIM-STORM Microscopy (O. Burri, T. Laroche, R. Guiet, A. Seitz)....Pages 95-103 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples (Patricia Bondia, Santiago Casado, Cristina Flors)....Pages 105-113 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles (Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann)....Pages 115-126 Two-Color Single-Molecule Tracking in Live Cells (Siegfried Hänselmann, Dirk-Peter Herten)....Pages 127-138 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy (Jan Philipp Eberle, Walter Muranyi, Holger Erfle, Manuel Gunkel)....Pages 139-152 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy (Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, Erik M. Jorgensen)....Pages 153-162 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells (Errin Johnson, Rainer Kaufmann)....Pages 163-177 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments (Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis)....Pages 179-188 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step (Susanna Maria Früh, Ingmar Schoen)....Pages 189-203 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy (Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman)....Pages 205-217 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells (Kem A. Sochacki, Justin W. Taraska)....Pages 219-230 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT (Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang et al.)....Pages 231-252 Super-Resolution High Content Screening and Analysis (K. Soliman)....Pages 253-259 Back Matter ....Pages 261-262