ENGLISH

ENZYMES: Catalysis, Kinetics and Mechanisms

By N.S. Punekar

Book information

Publisher
Springer Singapore
Year
2018
ISBN
978-981-13-0784-3, 978-981-13-0785-0
Language
english
Format
PDF
Filesize
16 MB (16630656 bytes)
Series
Eğitim Tanrısı
Edition
1st ed.
Pages
XXIII, 562\560
Time added
2019-01-12 08:10:22
Life Sciences; Enzymology; Protein-Ligand Interact

Description

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject. Front Matter ....Pages i-xxiii Front Matter ....Pages 1-1 Enzymes: Their Place in Biology (N. S. Punekar)....Pages 3-4 Enzymes: Historical Aspects (N. S. Punekar)....Pages 5-13 Exploiting Enzymes: Technology and Applications (N. S. Punekar)....Pages 15-31 On Enzyme Nomenclature and Classification (N. S. Punekar)....Pages 33-41 Hallmarks of an Enzyme Catalyst (N. S. Punekar)....Pages 43-51 Origins of Enzyme Catalytic Power (N. S. Punekar)....Pages 53-70 Which Enzyme Uses What Tricks? (N. S. Punekar)....Pages 71-74 Structure and Catalysis: Conformational Flexibility and Protein Motion (N. S. Punekar)....Pages 75-82 Front Matter ....Pages 83-83 Chemical Kinetics: Fundamentals (N. S. Punekar)....Pages 85-96 Concepts of Equilibrium and Steady State (N. S. Punekar)....Pages 97-106 ES Complex and Pre-steady-state Kinetics (N. S. Punekar)....Pages 107-114 Principles of Enzyme Assays (N. S. Punekar)....Pages 115-129 Good Kinetic Practices (N. S. Punekar)....Pages 131-142 Quantification of Catalysis and Measures of Enzyme Purity (N. S. Punekar)....Pages 143-153 Henri–Michaelis–Menten Equation (N. S. Punekar)....Pages 155-176 More Complex Rate Expressions (N. S. Punekar)....Pages 177-191 Enzyme Kinetic Data: Collection and Analysis (N. S. Punekar)....Pages 193-211 Front Matter ....Pages 213-213 Approaches to Kinetic Mechanism: An Overview (N. S. Punekar)....Pages 215-219 Analysis of Initial Velocity Patterns (N. S. Punekar)....Pages 221-230 Enzyme Inhibition Analyses (N. S. Punekar)....Pages 231-236 Irreversible Inhibitions (N. S. Punekar)....Pages 237-244 Reversible Inhibitions (N. S. Punekar)....Pages 245-257 Alternate Substrate (Product) Interactions (N. S. Punekar)....Pages 259-266 pH Studies with Enzymes (N. S. Punekar)....Pages 267-274 Isotopes in Enzymology (N. S. Punekar)....Pages 275-279 Isotope Exchanges at Equilibrium (N. S. Punekar)....Pages 281-286 Isotope Effects in Enzymology (N. S. Punekar)....Pages 287-299 From Kinetic Data to Mechanism and Back (N. S. Punekar)....Pages 301-310 Front Matter ....Pages 311-311 Chemical Reactivity and Molecular Interactions (N. S. Punekar)....Pages 313-330 Acid–Base Chemistry and Catalysis (N. S. Punekar)....Pages 331-343 Nucleophilic Catalysis and Covalent Reaction Intermediates (N. S. Punekar)....Pages 345-365 Phosphoryl Group Chemistry and Importance of ATP (N. S. Punekar)....Pages 367-383 Enzymatic Oxidation–Reduction Reactions (N. S. Punekar)....Pages 385-402 Carboxylations and Decarboxylations (N. S. Punekar)....Pages 403-420 Electrophilic Catalysis and Amino Acid Transformations (N. S. Punekar)....Pages 421-436 Integrating Kinetic and Chemical Mechanisms: A Synthesis (N. S. Punekar)....Pages 437-457 Front Matter ....Pages 459-459 Regulation of Enzyme Activity (N. S. Punekar)....Pages 461-492 In Vitro Versus In Vivo: Concepts and Consequences (N. S. Punekar)....Pages 493-519 Future of Enzymology: An Appraisal (N. S. Punekar)....Pages 521-551 Closure – Whither Enzymology (N. S. Punekar)....Pages 553-557 Back Matter ....Pages 559-562

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