ENGLISH

Transgenesis Techniques: Principles and Protocols

By Leonie Ringrose (auth.) , Elizabeth J. Cartwright (eds.)

Book information

Publisher
Humana Press
Year
2009
ISBN
978-1-60327-018-2, 978-1-60327-019-9
DOI
10.1007/978-1-60327-019-9
Language
english
Format
PDF
Filesize
4 MB (4268732 bytes)
Series
Methods in Molecular Biology 561
Edition
3
Pages
335\330
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Human Genetics; Animal Genetics and Genomics; Tran

Description

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.

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