ENGLISH

PCR Sequencing Protocols

By Ralph Rapley

Book information

Publisher
Humana Press
Year
1996
ISBN
9780896033443, 0896033449
ASIN
B001DABOT2
LCC
QP606.D46 P365 1996
Google Books ID
diMJsuAUB7EC
Open Library ID
OL989435M
Language
english
Format
PDF
Filesize
14 MB (14914011 bytes)
Series
Methods in Molecular Biology 065
Edition
1st
Pages
218\218
Topic
Biology
Library
mexmat
Scanned
no
Time added
2009-07-20 03:45:11

Description

A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.

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