Circulating tumor cells : methods and protocols

Description

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Circula ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs. Read more... Abstract: This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Read more... Front Matter ....Pages i-xxv Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin (Chian-Hui Lai, Ying-Chih Chang)....Pages 1-19 Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability (Emily S. Park, Simon P. Duffy, Hongshen Ma)....Pages 21-32 A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples (Jose I. Varillas, Kangfu Chen, Jinling Zhang, Thomas J. George Jr., Z. Hugh Fan)....Pages 33-53 Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis (Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang)....Pages 55-64 Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells (Soojung Claire Hur, James Che, Dino Di Carlo)....Pages 65-79 Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells (Sijie Hao, Merisa Nisic, Hongzhang He, Yu-Chong Tai, Si-Yang Zheng)....Pages 81-91 Microfilter-Based Capture and Release of Viable Circulating Tumor Cells (Siddarth Rawal, Zheng Ao, Ram H. Datar, Ashutosh Agarwal)....Pages 93-105 Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration (Anthony Williams, Jaebum Chung, Changhuei Yang, Richard J. Cote)....Pages 107-117 Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood (Michael Pugia, Mark Jesus M. Magbanua, John W. Park)....Pages 119-131 Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells (Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace)....Pages 133-141 Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification (Nisha Kanwar, Susan J. Done)....Pages 143-152 Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter) (Chin Chun Ooi, Seung-min Park, Dawson J. Wong, Sanjiv S. Gambhir, Shan X. Wang)....Pages 153-162 RareCyte® CTC Analysis Step 1: AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood (Arturo B. Ramirez, Lance U’Ren, Daniel E. Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell et al.)....Pages 163-172 RareCyte® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semiautomated Image Analysis (Jeffrey L. Werbin, Joshua J. Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L. Stilwell, Eric P. Kaldjian)....Pages 173-180 RareCyte® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis (Jackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre et al.)....Pages 181-192 Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells (Stephanie S. Yee, Erica L. Carpenter)....Pages 193-202 Enumeration of Circulating Tumor Cells and Disseminated Tumor Cells in Blood and Bone Marrow by Immunomagnetic Enrichment and Flow Cytometry (IE/FC) (Mark Jesus M. Magbanua, Tulasi I. Solanki, Andrea D. Ordonez, Feng Hsiao, John W. Park)....Pages 203-210 Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR) (Tomoyuki Okumura, Tetsuji Yamaguchi, Toru Watanabe, Takuya Nagata, Yutaka Shimada)....Pages 211-217 Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood Samples (Brandon Miller, Maryam Lustberg, Thomas A. Summers, Jeffrey J. Chalmers)....Pages 219-234 Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells (Zheng Ao, Xiaohe Liu)....Pages 235-246 A Noninvasive and Real-Time Method for Circulating Tumor Cell Detection by In Vivo Flow Cytometry (Xunbin Wei, Jian Zhou, Xi Zhu, Xinrong Yang, Ping Yang, Qiyan Wang)....Pages 247-262 EpCAM-Independent Enrichment and Detection of Viable Circulating Tumor Cells Using the EPISPOT Assay (Alexandra Soler, Laure Cayrefourcq, Martine Mazel, Catherine Alix-Panabières)....Pages 263-276 Utilizing Matrigel Transwell Invasion Assay to Detect and Enumerate Circulating Tumor Cells (Xingtong Liu, Xiangwei Wu)....Pages 277-282 Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection (M. J. Alvarez Cubero, J. A. Lorente, I. Robles-Fernandez, A. Rodriguez-Martinez, J. L. Puche, M. J. Serrano)....Pages 283-303 Back Matter ....Pages 305-306