ENGLISH

Electrotransformation of Bacteria

By Natalie Eynard , Justin Teissié (auth.) , Natalie Eynard PhD , Justin Teissié (eds.)

Book information

Publisher
Springer-Verlag Berlin Heidelberg
Year
2000
ISBN
978-3-642-08593-2, 978-3-662-04305-9
DOI
10.1007/978-3-662-04305-9
Language
english
Format
PDF
Filesize
4 MB (4701120 bytes)
Series
Springer Lab Manuals
Edition
1
Pages
292\296
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Biotechnology; Microbiology; Molecular Medicine; C

Description

Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduced genetic material into bacterial cells, with the use of a strong electric field. In the present manual, protocols for the transformation of about 40 strains of bacteria are described. Emphasis is given on the individual critical procedural steps, since the practical details depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows the user to modify and adapt each protocol to his/her own experiments. Bacterial strains with relevance in the food industry and biotechnology, in medical and veterinary fields, agroindustry and environmental sciences are covered.

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