ENGLISH

RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay

Book information

Publisher
Academic Press
Year
2008
ISBN
0123743788, 9780123743787
ISSN
0076-6879
Language
english
Format
PDF
Filesize
11 MB (11214264 bytes)
Series
Methods in Enzymology 448
Edition
1
Pages
656\619
Scanned
yes
Time added
2012-03-09 12:00:00

Description

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The control of biological processes, such as cellular growth and differentiation, is dependent on how the genetic material within a cell is expressed. The cellular physiology of mRNA-including mRNA processing, transport, localization, and turnover-is central to the process of gene expression. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression.Offers step-by-step lab instructions including necessary equipment and reagents

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