Technology platforms for 3D cell culture : a user's guide

Description

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. -Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture -Features contributions from leading developers and researchers active in 3D cell technology -Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied -Includes information on resources and technical support to help initiate the use of 3D culture methods. Read more... Abstract: Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Read more... Content: Title Page Copyright Page Contents List of contributors Preface List of abbreviations Chapter 1 An introduction to the third dimension for routine cell culture Introduction Aggregate-based technologies Scaffold-based technologies 3D bioreactors Barriers to adoption and future directions References Part I Aggregate-based technologies Chapter 2 Gravity-enforced microtissue engineering Introduction Product description Tumour microtissue size profiling for drug testing Conclusions Troubleshooting and notes (Table 2.3) Summary References. Chapter 3 Physiologically relevant spheroid models for three-dimensional cell culture Introduction Description of technology Experimental results obtained using the Perfecta3D hanging drop plates Troubleshooting Conclusion References Chapter 4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture Introduction Description of technology Example of application Troubleshooting Summary References Chapter 5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues -- the 3D Petri Dish® IntroductionDescription of technology Applications Protocols Troubleshooting Conclusion References Chapter 6 Organotypic microtissues on an air-liquid interface Introduction Description of technology 3D neural OrganDOT" tissues for neurotoxicology studies Troubleshooting References Part II Hydrogels Chapter 7 Materials and assay systems used for three?dimensional cell culture Introduction Description of technology Applications of 3D cultures in vitro Conclusion References Chapter 8 HyStem®, a customisable hyaluronan?based hydrogel matrix for 3D cell culture Introduction. Description of technologyApplications Troubleshooting Conclusion Appendix References Chapter 9 3-D Life biomimetic hydrogels: A modular system for cell environment design Introduction Applications Description of technology A tumour-stroma model: 3D co-culture of MCF-7 cells and primary fibroblasts Troubleshooting Conclusion References Part III Scaffolds Chapter 10 Alvetex®, a highly porous polystyrene scaffold for routine three-dimensional cell culture Introduction Description of technology Example applications of Alvetex Scaffold technology Troubleshooting Conclusion.