Neisseria meningitidis: Methods and Protocols
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Description
This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration and consistency in investigations of this enigmatic and dangerous pathogen. Front Matter ....Pages i-xiii An Overview of Neisseria meningitidis (Sarah Hollingshead, Christoph M. Tang)....Pages 1-16 Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR (Ala-Eddine Deghmane, Eva Hong, Muhamed-Kheir Taha)....Pages 17-31 Investigating RNA–Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis (Nadja Heidrich, Saskia Bauriedl, Christoph Schoen)....Pages 33-49 Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics (Holly B. Bratcher, Odile B. Harrison, Martin C. J. Maiden)....Pages 51-82 Determination of Repeat Number and Expression States of Phase-Variable Loci Through Next Generation Sequencing and Bioinformatic Analysis (Luke R. Green, Richard D. Haigh, Christopher D. Bayliss)....Pages 83-92 Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis (Freda E. -C. Jen, Kate L. Seib, Aimee Tan)....Pages 93-104 Sample Preparation for Proteomic Analysis of Neisseria meningitidis (Benjamin L. Schulz)....Pages 105-112 Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance (Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Christopher J. Day, Michael P. Jennings, Kate L. Seib)....Pages 113-121 The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp. (Emily A. Kibble, Mitali Sarkar-Tyson, Geoffrey W. Coombs, Charlene M. Kahler)....Pages 123-133 In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells (Brandon J. Kim, Alexandra Schubert-Unkmeir)....Pages 135-148 Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease (Kay O. Johswich, Scott D. Gray-Owen)....Pages 149-168 Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays (Jay Lucidarme, Jennifer Louth, Kelly Townsend-Payne, Ray Borrow)....Pages 169-179 Monoclonal Antibodies Against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitidis: A Platform for the Quality Control of Meningococcal Vaccines (Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez et al.)....Pages 181-203 Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine (Giuseppe Boccadifuoco, Brunella Brunelli, Elena Mori, Mauro Agnusdei, Claudia Gianfaldoni, Marzia Monica Giuliani)....Pages 205-215 Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitidis (Jakob Loschko, Karen Garcia, David Cooper, Michael Pride, Annaliesa Anderson)....Pages 217-236 Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis (Caroline Cayrou, Christopher D. Bayliss)....Pages 237-249 Back Matter ....Pages 251-253
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