ENGLISH

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis

By Marie A. Di Berardino (auth.) , Paul J. Verma , Alan O. Trounson (eds.)

Book information

Publisher
Humana Press
Year
2006
ISBN
978-1-58829-280-3, 978-1-59745-154-3
DOI
10.1007/978-1-59745-154-3
Language
english
Format
PDF
Filesize
3 MB (3096228 bytes)
Series
Methods in Molecular Biology™ 348
Edition
1
Pages
338\331
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Cell Biology

Description

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.

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