Streptococcus pneumoniae: Methods and Protocols

Description

This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field. Front Matter ....Pages i-xv Front Matter ....Pages 1-1 Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media (Norma Suárez, Esther Texeira)....Pages 3-10 Front Matter ....Pages 11-11 Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell (Sven Hammerschmidt, Manfred Rohde)....Pages 13-33 Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell (Federico Iovino, Birgitta Henriques-Normark)....Pages 35-39 Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome (Morten Kjos)....Pages 41-51 High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host (Federico Iovino, Birgitta Henriques-Normark)....Pages 53-59 Front Matter ....Pages 61-61 Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome (Isabelle Mortier-Barrière, Nathalie Campo, Mathieu A. Bergé, Marc Prudhomme, Patrice Polard)....Pages 63-78 Gene Expression Analysis in the Pneumococcus (Rory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, N. Luisa Hiller)....Pages 79-88 Transcriptional Knockdown in Pneumococci Using CRISPR Interference (Morten Kjos)....Pages 89-98 Front Matter ....Pages 99-99 Protein Expression Analysis by Western Blot and Protein–Protein Interactions (María Dolores Cima-Cabal, Fernando Vazquez, Juan R. de los Toyos, María del Mar García-Suárez)....Pages 101-111 Mass Spectrometry to Study the Bacterial Proteome from a Single Colony (Jianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng)....Pages 113-121 Bead-Based Flow-Cytometric Cell Counting of Live and Dead Bacteria (Fang Ou, Cushla McGoverin, Joni White, Simon Swift, Frédérique Vanholsbeeck)....Pages 123-134 Front Matter ....Pages 135-135 In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumoniae with Host Cells (Terry Brissac, Carlos J. Orihuela)....Pages 137-146 Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae (Yashuan Chao, Caroline Bergenfelz, Anders P. Hakansson)....Pages 147-171 In Vivo Mouse Models to Study Pneumococcal Host Interaction and Invasive Pneumococcal Disease (Federico Iovino, Vicky Sender, Birgitta Henriques-Normark)....Pages 173-181 Two-Photon Intravital Imaging of Leukocytes in the Trachea During Pneumococcal Infection (Miguel Palomino-Segura, Santiago F. Gonzalez)....Pages 183-194 IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo (Adam Sierakowiak, Birgitta Henriques-Normark, Federico Iovino)....Pages 195-202 Front Matter ....Pages 203-203 The Pneumococcus and Its Critical Role in Public Health (Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani)....Pages 205-213 The Epidemiology and Biostatistics of Pneumococcus (Godwin Oligbu, Norman K. Fry, Shamez N. Ladhani)....Pages 215-224 Back Matter ....Pages 225-227