ENGLISH

RT-PCR Protocols

By Nicola King , Joe O'Connell

Book information

Publisher
Humana Press
Year
2002
ISBN
0896038750, 9780896038752
Open Library ID
OL9864731M
Language
english
Format
PDF
Filesize
4 MB (4598555 bytes)
Series
Methods in Molecular Biology
Edition
1st
Pages
394\394
Topic
Biology
Library
demonoid.me
Time added
2011-04-11 19:55:47

Description

A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

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