ENGLISH

Monoclonal Antibody Protocols

Book information

Publisher
Humana Press
Year
1995
ISBN
9780896033085, 0896033082
LCC
QR186.85 .M66285 1995
Open Library ID
OL781589M
Language
english
Format
PDF
Filesize
15 MB (15644895 bytes)
Series
Methods in Molecular Biology, Vol 45
Edition
1
Pages
260\260
Topic
Biology
Library
mexmat
Scanned
no
Time added
2009-07-20 03:45:11

Description

Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.

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