ENGLISH

Plant Stress Tolerance : Methods and Protocols

By Sunkar , Ramanjulu

Book information

Year
2017
ISBN
978-1-4939-7136-7, 1493971360, 978-1-4939-7134-3
Language
english
Format
PDF
Filesize
10 MB (10073574 bytes)
Series
Methods in Molecular Biology 1631
Edition
2nd ed.
Pages
\354
Time added
2017-10-15 16:00:00

Description

This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Epigenetics and RNA Processing: Connections to Drought, Salt, and ABA? (Min May Wong, Geeng Loo Chong, Paul E. Verslues)....Pages 3-21 The Fundamental Role of Reactive Oxygen Species in Plant Stress Response (Michael Liebthal, Karl-Josef Dietz)....Pages 23-39 The Role of Long Noncoding RNAs in Plant Stress Tolerance (Yuepeng Song, Deqiang Zhang)....Pages 41-68 Toward a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture (Venkatachalam Lakshmanan, Prasun Ray, Kelly D. Craven)....Pages 69-84 Front Matter ....Pages 85-85 Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation (Thorsten Seidel, Derya Kirasi)....Pages 87-107 Generation of a Stress-Inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-Responsive Gene Deregulation Mutants (Si-in Yu, Byeong-ha Lee)....Pages 109-119 Detection of Differential DNA Methylation Under Stress Conditions Using Bisulfite Sequence Analysis (Ibtisam Al Harrasi, Rashid Al-Yahyai, Mahmoud W. Yaish)....Pages 121-137 ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants (Guosheng Li, Guru Jagadeeswaran, Andrew Mort, Ramanjulu Sunkar)....Pages 139-149 Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants (Yong-Fang Li, Ramamurthy Mahalingam, Ramanjulu Sunkar)....Pages 151-161 Global Proteomic Profiling and Identification of Stress-Responsive Proteins Using Two-Dimensional Gel Electrophoresis (Pragya Barua, Dipak Gayen, Nilesh Vikram Lande, Subhra Chakraborty, Niranjan Chakraborty)....Pages 163-179 Phosphoproteomics Analysis for Probing Plant Stress Tolerance (Christof Rampitsch)....Pages 181-193 Probing Posttranslational Redox Modifications (Patrick Treffon, Michael Liebthal, Wilena Telman, Karl-Josef Dietz)....Pages 195-219 Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants (Ashwini R. Jamdhade, Ramanjulu Sunkar, Vandana K. Hivrale)....Pages 221-227 Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays for SO, APR, SiR, and In-Gel SiR Activity (Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky et al.)....Pages 229-251 Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants (Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky et al.)....Pages 253-271 Determining Glutathione Levels in Plants (Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, Sanjib Kumar Panda)....Pages 273-277 Porous Graphitic Carbon Liquid Chromatography–Mass Spectrometry Analysis of Drought Stress-Responsive Raffinose Family Oligosaccharides in Plant Tissues (Tiago F. Jorge, Maria H. Florêncio, Carla António)....Pages 279-293 Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses (Suhas Shinde, Shivakumar Devaiah, Aruna Kilaru)....Pages 295-303 Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC) (Xiaoqing Gong, Ji-Hong Liu)....Pages 305-311 Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis (Gounipalli Veeranagamallaiah, Chinta Sudhakar)....Pages 313-323 Rapid Quantification of Abscisic Acid by GC-MS/MS for Studies of Abiotic Stress Response (Paul E. Verslues)....Pages 325-335 Silencing of Stress-Regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach (Sachin Teotia, Guiliang Tang)....Pages 337-348 Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes (Venkatachalam Lakshmanan, Prasun Ray, Kelly D. Craven)....Pages 349-362 Back Matter ....Pages 363-365

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