ENGLISH

Baculovirus and Insect Cell Expression Protocols

By David W. Murhammer (auth.) , David W. Murhammer (eds.)

Book information

Publisher
Humana Press
Year
2007
ISBN
9781588295378, 1588295370
DOI
10.1007/978-1-59745-457-5
Open Library ID
OL17233848M
Language
english
Format
PDF
Filesize
3 MB (3626264 bytes)
Series
Methods in Molecular Biology 388
Edition
2
Pages
470\446
Topic
Biology
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Biochemistrygeneral; Plant Biochemistry; Cell Biology; Plant S

Description

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

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