ENGLISH

Kidney Organogenesis: Methods and Protocols

By Seppo Vainio

Book information

Publisher
Springer New York;Humana Press
Year
2019
ISBN
978-1-4939-9020-7, 978-1-4939-9021-4
Language
english
Format
PDF
Filesize
7 MB (7739649 bytes)
Series
Methods in Molecular Biology 1926
Edition
1st ed.
Pages
XI, 248\242
Time added
2019-04-19 19:00:00
Biomedicine; Molecular Medicine; Cell Culture; Nep

Description

This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development (Frank Costantini)....Pages 3-21 Mouse Ex Vivo Kidney Culture Methods (Anneliis Ihermann-Hella, Satu Kuure)....Pages 23-30 Serum-Free Organ Culture of the Embryonic Mouse Ureter (Filipa M. Lopes, Adrian S. Woolf)....Pages 31-38 Experimental Tubulogenesis Induction Model in the Mouse (Aleksandra Rak-Raszewska)....Pages 39-51 Front Matter ....Pages 53-53 Dissociation of Tissues for Single-Cell Analysis (Andrew S. Potter, S. Steven Potter)....Pages 55-62 Long-Term Culture of Nephron Progenitor Cells Ex Vivo (Aaron C. Brown, Ashwani K. Gupta, Leif Oxburgh)....Pages 63-75 3D Cell Culture Models of Epithelial Tissues (Kai Zhang, Aki Manninen)....Pages 77-84 Front Matter ....Pages 85-85 Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells (Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura)....Pages 87-102 Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells (Krithika Hariharan, Petra Reinke, Andreas Kurtz)....Pages 103-115 Protocol to Generate Ureteric Bud Structures from Human iPS Cells (Shin-Ichi Mae, Makoto Ryosaka, Kenji Osafune)....Pages 117-123 Front Matter ....Pages 125-125 Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture (Melanie L. Lawrence, Mona Elhendawi, Jamie A. Davies)....Pages 127-142 Virus as Renal Functional Genetic Tools (Abhishek Sharma, Sandhanakrishnan Cattavarayane)....Pages 143-149 Gene Editing in 3D Cultured Nephron Progenitor Cell Lines (Zhongwei Li, Toshikazu Araoka, Juan Carlos Izpisua Belmonte)....Pages 151-159 Front Matter ....Pages 161-161 Time-Lapse Technologies and 4D Imaging of Kidney Development (Ulla Saarela, Ilya Skovorodkin)....Pages 163-167 Decellularizing and Recellularizing Adult Mouse Kidneys (Theresa Chow, Jennifer Whiteley, Ian M. Rogers)....Pages 169-184 Optical Projection Tomography Imaging to Study Kidney Organogenesis (Renata Prunskaite-Hyyryläinen)....Pages 185-199 Front Matter ....Pages 201-201 Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model (Shireen-A. Davies, Pablo Cabrero, Richard Marley, Guillermo Martinez Corrales, Saurav Ghimire, Anthony J. Dornan et al.)....Pages 203-221 Analysis of the Teashirt Target Genes in Ureteric Bud Development (Laurent Fasano, Irene Sanchez-Martin, Xavier Caubit)....Pages 223-232 Front Matter ....Pages 233-233 Methods of Computational Analysis in Kidney Development (Pauli Tikka, Franz Schaefer)....Pages 235-246 Back Matter ....Pages 247-248

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