ENGLISH

Rat Genomics

By G. Thomas Hayman , Jennifer R. Smith , Melinda R. Dwinell , Mary Shimoyama

Book information

Publisher
Springer New York; Humana
Year
2019
ISBN
978-1-4939-9580-6;978-1-4939-9581-3
Language
english
Format
PDF
Filesize
11 MB (11046882 bytes)
Series
Methods in Molecular Biology 2018
Edition
1st ed.
Pages
XV, 331\335
Time added
2019-09-18 12:17:02
Life Sciences; Animal Genetics and Genomics; Anima

Description

This book provides both historical perspective and practical information to support researchers either currently involved in genomic research on rats or planning to begin such a project. In numerous chapters, a detailed protocol is provided for researchers looking to move into a new area of investigation or to leverage a new technology. In other cases, a detailed review of existing models or a description of available resources can help the researcher find, understand, and utilize the information, the data, and the tools that they need to support their research efforts. Written as part of the highly successful Methods in Molecular Biology series, this collection includes the kind of hands-on detail necessary for success in the lab. Authoritative and up-to-date, Rat Genomics explores the rat as a biomedical model uniquely poised to provide the ideal combination of established experimental models, extensive physiological data, and genomic manipulability to facilitate exploration of the underlying biology. Front Matter ....Pages i-xv The Rat: A Model Used in Biomedical Research (Jennifer R. Smith, Elizabeth R. Bolton, Melinda R. Dwinell)....Pages 1-41 Rat Genome Assemblies, Annotation, and Variant Repository (Monika Tutaj, Jennifer R. Smith, Elizabeth R. Bolton)....Pages 43-70 Rat Genome Databases, Repositories, and Tools (Stanley J. F. Laulederkind, G. Thomas Hayman, Shur-Jen Wang, Matthew J. Hoffman, Jennifer R. Smith, Elizabeth R. Bolton et al.)....Pages 71-96 Next Generation Transgenic Rat Model Production (Wanda E. Filipiak, Elizabeth D. Hughes, Galina B. Gavrilina, Anna K. LaForest, Thomas L. Saunders)....Pages 97-114 Embryonic Stem Cells and Gene Manipulation in Rat (Masumi Hirabayashi, Akiko Takizawa, Shinichi Hochi)....Pages 115-130 Protocols for Cryopreservation and Rederivation of Rat Gametes (Akiko Takizawa, Tomoo Eto)....Pages 131-149 Fluorescent Imaging and Microscopy for Dynamic Processes in Rats (Ruben M. Sandoval, Bruce A. Molitoris, Oleg Palygin)....Pages 151-175 Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Adapter (Yong Liu, Alison J. Kriegel, Mingyu Liang)....Pages 177-194 Characterization of the Rat Gut Microbiota via 16S rRNA Amplicon Library Sequencing (Aaron C. Ericsson, Susheel B. Busi, James M. Amos-Landgraf)....Pages 195-212 Networking in Biology: The Hybrid Rat Diversity Panel (Boris Tabakoff, Harry Smith, Lauren A. Vanderlinden, Paula L. Hoffman, Laura M. Saba)....Pages 213-231 Using Heterogeneous Stocks for Fine-Mapping Genetically Complex Traits (Leah C. Solberg Woods, Abraham A. Palmer)....Pages 233-247 Mapping Mammary Tumor Traits in the Rat (Michael J. Flister, Amit Joshi, Carmen Bergom, Hallgeir Rui)....Pages 249-267 Rat Models of Metabolic Syndrome (Anne E. Kwitek)....Pages 269-285 Genomic Research in Rat Models of Kidney Disease (Yoram Yagil, Ronen Levi-Varadi, Chana Yagil)....Pages 287-307 Rat Models of Exercise for the Study of Complex Disease (Lauren Gerard Koch, Steven L. Britton)....Pages 309-317 Behavioral Genetic Studies in Rats (Yangsu Ren, Abraham A. Palmer)....Pages 319-326 Back Matter ....Pages 327-331

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