ENGLISH

Recombinant DNA technology and genetic engineering

By K. Rajagopal

Book information

Publisher
Tata McGraw Hill Education Private Limited
Year
2012
ISBN
9780071077798, 0071077790, 9781259006555, 1259006557
Language
english
Format
PDF
Filesize
61 MB (64117542 bytes)
Pages
\342
Time added
2020-04-28 15:01:29

Description

Cover Contents 1. Introduction to Gene Cloning Introduction to Gene Cloning and Genetic Engineering What is Gene Cloning? Gene Cloning and PCR 2. Salient Features of Cloning Vectors Essential Components of Gene Cloning Conjugative and Nonconjugative Plasmids Commercially Available Plasmids Cosmids Preparation of Genomic DNA Libraries in Cosmid Vectors ssDNA Phages Filamentous Phage Phagemid Vectors Yeast Cloning Vectors Cloning Vectors for Higher Organisms Direct Transfer or Integration Vectors 3. Plasmid Biology Structural and Functional Organisation of Plasmids Characteristics of Plasmids Plasmid Replication Plasmids Carrying Conjugation or Fertility Factors Different Types of Plasmids 4. Enzymes Used in Genetic Engineering Introduction DNA Polymerase Reverse Transcriptase Terminal Deoxynucleotidyl Transferase RNA Polymerases Addition of Phosphate Group/Kinasing Dephosphorelation by Alkaline Phosphatase Ligases Restriction Endonuclease I and II Nick Translation Probe Synthesis from Phagemid 5. Isolation of Genomic and Nuclear DNA Introduction DNA Sequencing–To Understand the Structure of a Gene Sequencing Gel PCR Product Sequencing 6. Cloning and Subcloning Strategy Construction of Recombinant DNA Diagrammatic Representation of Different Cloning Technologies Preparation of Competent Cell-Transformation, Transfection Insertional Inactivation (The Other Way of Screening the Clones) Transformation of Double-Stranded DNA Recombinant Selection and Screening Essential Tools Required for Cloning/Subcloning 7. Selection of rDNA Clones and Their Expression Products Introduction Selection Analysis of cDNA Library Preparation of Polyclonal Antibody ELISA (Enzyme Linked Immunosorbent Assay) Western Blotting SDS – PAGE Hybridisation Basic Mechanism of Hybridisation 8. DNA Technology Mutagenesis Oligonucleotide-based Mutagenesis PCR-based Site Directed Mutagenesis Polymerase Chain Reaction (PCR) Applications of rDNA Technology in Diagnostics rDNA Technology and Forensics DNA Fingerprinting for Sex Determination 9. The Application of Gene Cloning and DNA Analysis Production of Protein from Cloned Genes Cloning and Expression Vectors for System E. Coli Promoters for Gene Expression and Protein Production Recombinant Protein Production in : General Problems Eukaryotes for Protein Expression and Production Recombinant Protein Production in Pichia Pastoris, Saccharomyces Cerevisiae and Kluveromyces Lactis Filamentous Fungi for Protein Expression and Production Gene Cloning and DNA Analysis in Medicine Eukaryotic Gene Synthesis and Expression in Synthesis, Cloning and Expression of other Recombinant Proteins with Therapeutic Importance Gene Therapy Gene Subtraction/Antisense Technology Glossary Index

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