Biobanking: Methods and Protocols

Description

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies. Front Matter ....Pages i-xvi Sustainability in Biobanking (Maram Abdaljaleel, Elyse J. Singer, William H. Yong)....Pages 1-6 An Introduction to Starting a Biobank (Mitra D. Harati, Ryan R. Williams, Masoud Movassaghi, Amin Hojat, Gregory M. Lucey, William H. Yong)....Pages 7-16 An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks (Kyuseok Im, Dorina Gui, William H. Yong)....Pages 17-29 Disaster Prevention and Recovery (Chon Boon Eng, Wei Ling Tan)....Pages 31-41 Minority Participation in Biobanks: An Essential Key to Progress (Paula Kim, Erin L. Milliken)....Pages 43-50 Orientation and Training of New Biobank Personnel (Ryan R. Williams, Diviya Gupta, William H. Yong)....Pages 51-63 Procurement and Storage of Surgical Biospecimens (Amin Hojat, Bowen Wei, Madeline G. Olson, Qinwen Mao, William H. Yong)....Pages 65-76 Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization (Randy S. Tashjian, Ryan R. Williams, Harry V. Vinters, William H. Yong)....Pages 77-87 Procurement, Storage, and Use of Blood in Biobanks (Jaclyn N. Perry, Afreen Jasim, Amin Hojat, William H. Yong)....Pages 89-97 Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens (Jennifer S. Woo, David Y. Lu)....Pages 99-105 Biobanking of Cerebrospinal Fluid (Randy S. Tashjian, Harry V. Vinters, William H. Yong)....Pages 107-114 Biobanking of Urine Samples (Neda A. Moatamed)....Pages 115-124 Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking (Alberto M. Marchevsky, Shikha Bose, Beatrice Knudsen)....Pages 125-133 Fluid Preservation of Zoological Specimens (Thomas P. V. Hartman)....Pages 135-148 Photographing Fluid-Preserved Specimens (David Glynne Fox, Thomas P. V. Hartman)....Pages 149-153 Casts of Fluid Preserved Specimens (Sarah Burhouse, Thomas P. V. Hartman)....Pages 155-162 Collection and Preservation of Terrestrial Arthropods (Jeffrey D. Whitman, Douglas Yanega, Cole B. G. Watson, Vincent W. Strode)....Pages 163-189 Procurement and Preservation of Plants (Allison D. Rudalevige, Jeffrey D. Whitman)....Pages 191-212 Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank (Lisa Ta, Laura Gosa, David A. Nathanson)....Pages 213-225 Decontamination of Biobank Facilities (Xinhai Zhang, Saied Mirshahidi, Chien-Shing Chen)....Pages 227-241 Laboratory Safety: Chemical and Physical Hazards (Saeed Asiry, Lee-Cyn Ang)....Pages 243-252 Making Formalin-Fixed, Paraffin Embedded Blocks (Alireza Sadeghipour, Pegah Babaheidarian)....Pages 253-268 Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections (Joanne Sy, Lee-Cyn Ang)....Pages 269-278 Performing and Cutting Frozen Sections (Ramir S. Arcega, Jennifer S. Woo, Haodong Xu)....Pages 279-288 An Introduction to the Performance of Immunohistochemistry (Shino Magaki, Seyed A. Hojat, Bowen Wei, Alexandra So, William H. Yong)....Pages 289-298 An Introduction to Performing Immunofluorescence Staining (Kyuseok Im, Sergey Mareninov, M. Fernando Palma Diaz, William H. Yong)....Pages 299-311 Making a Tissue Microarray (Matthew Koo, Jill M. Squires, Daphne Ying, Jiaoti Huang)....Pages 313-323 Nucleic Acid Isolation and Quality Control (Ling Dong, Janice Yoshizawa, Xinmin Li)....Pages 325-343 Fundamentals of RNA Analysis on Biobanked Specimens (Samuel P. Strom)....Pages 345-357 Nucleic Acid Extraction from Human Biological Samples (Sureni V. Mullegama, Michael O. Alberti, Cora Au, Yan Li, Traci Toy, Vanina Tomasian et al.)....Pages 359-383 An Overview of DNA Analytical Methods (Valerie A. Arboleda, Rena R. Xian)....Pages 385-402 Shotgun Proteomic Profiling of Bloodborne Nanoscale Extracellular Vesicles (Pete Heinzelman, David N. Powers, James A. Wohlschlegel, Varghese John)....Pages 403-416 Sample Preparation for Transmission Electron Microscopy (Parastou Tizro, Cecilia Choi, Negar Khanlou)....Pages 417-424 Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines (Laura Gosa, Lisa Ta, David A. Nathanson)....Pages 425-431 Domestic and International Shipping of Biospecimens (Dominique Gordy, Randy S. Tashjian, Hannah Lee, Masoud Movassaghi, William H. Yong)....Pages 433-443 Back Matter ....Pages 445-448