Neisseria gonorrhoeae: Methods and Protocols

Description

This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as producing major gonococcal antigens to high purity, genetic transformation of gonococci and strategies for global RNA sequencing, evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function, as well as a number of methods and protocols for studying the biological interactions of the gonococcus and the host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Neisseria gonorrhoeae: Methods and Protocols provides researchers with a foundation for progress toward the eventual control of this disease and a significant reduction in global case numbers of gonorrhea. Front Matter ....Pages i-xvii Biology of the Gonococcus: Disease and Pathogenesis (Jutamas Shaughnessy, Sanjay Ram, Peter A. Rice)....Pages 1-27 How to Identify Exposed Women Who Are Infected with Neisseria gonorrhoeae (Stephanie E. McLaughlin, J. McLeod Griffiss)....Pages 29-36 Antimicrobial Resistance in Neisseria gonorrhoeae and Treatment of Gonorrhea (Magnus Unemo, Daniel Golparian, David W. Eyre)....Pages 37-58 Prediction of Minimum Inhibitory Concentrations of Antimicrobials for Neisseria gonorrhoeae Using Whole-Genome Sequencing (David W. Eyre, Daniel Golparian, Magnus Unemo)....Pages 59-76 Gonococcal Colony Typing (John E. Heckels)....Pages 77-85 Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae (Myron Christodoulides)....Pages 87-96 Purification of Type IV Pili and Pilin Subunits (Lisa Craig, Tuba Altindal)....Pages 97-110 Peptidoglycan Composition in Neisseria (Ryan E. Schaub, Joseph P. Dillard)....Pages 111-120 Isolation of Naturally Released Gonococcal Outer Membrane Vesicles as Vaccine Antigens (Ian P. Francis, Xiuping Lui, Lee M. Wetzler)....Pages 121-141 Transformation in Neisseria gonorrhoeae (Melanie M. Callaghan, Joseph P. Dillard)....Pages 143-162 Strategies for Global RNA Sequencing of the Human Pathogen Neisseria gonorrhoeae (Ryan McClure, Caroline A. Genco)....Pages 163-183 Bioinformatics Workflow for Gonococcal Proteomics (Fadi E. El-Rami, Aleksandra E. Sikora)....Pages 185-205 Phenotypic MicroArray Screening of Neisseria gonorrhoeae in Chemically Defined Liquid Medium (Benjamin I. Baarda, Aleksandra E. Sikora)....Pages 207-216 Generation of Metal-Depleted Conditions for In Vitro Growth of Neisseria gonorrhoeae (Cynthia Nau Cornelissen)....Pages 217-231 Cloning, Expression, and Purification of Recombinant Neisseria gonorrhoeae Proteins (María Victoria Humbert)....Pages 233-266 Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae (Sunita Gulati, Peter A. Rice, Sanjay Ram)....Pages 267-280 Basic Methods for Examining Neisseria gonorrhoeae Interactions with Host Cells In Vitro (Hannia Liliana Almonacid-Mendoza, Myron Christodoulides)....Pages 281-299 Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection (Jimena Gatica, Paula I. Rodas, Alejandro Escobar)....Pages 301-318 Protocols to Interrogate the Interactions Between Neisseria gonorrhoeae and Primary Human Neutrophils (Stephanie A. Ragland, Alison K. Criss)....Pages 319-345 Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model (Paweł Łaniewski, Melissa M. Herbst-Kralovetz)....Pages 347-361 In Vitro Models of Eye Infection with Neisseria gonorrhoeae (Colin P. Churchward, Lori A. S. Snyder)....Pages 363-376 Use of Human Fallopian Tube Organ in Culture (FTOC) and Primary Fallopian Tube Epithelial Cells (FTEC) to Study the Biology of Neisseria gonorrhoeae Infection (A. Said Álamos-Musre, Alejandro Escobar, Cecilia V. Tapia, Myron Christodoulides, Paula I. Rodas)....Pages 377-402 A Natural Mouse Model for Neisseria Persistent Colonization (Katherine Rhodes, Mancheong Ma, Magdalene So)....Pages 403-412 Female Mouse Model of Neisseria gonorrhoeae Infection (Erica L. Raterman, Ann E. Jerse)....Pages 413-429 Experimental Human Infection with Neisseria gonorrhoeae (Marcia M. Hobbs, Joseph A. Duncan)....Pages 431-452 Back Matter ....Pages 453-461