Basic Concepts in Clinical Biochemistry: A Practical Guide

Description

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance. Front Matter ....Pages i-xxii Common Clinical Laboratory Hazards and Waste Disposal (Vijay Kumar, Kiran Dip Gill)....Pages 1-3 Blood Collection and Preservation (Vijay Kumar, Kiran Dip Gill)....Pages 5-7 Quality Control in Laboratory (Vijay Kumar, Kiran Dip Gill)....Pages 9-12 Automation in Clinical Laboratory (Vijay Kumar, Kiran Dip Gill)....Pages 13-15 Photometry: Colorimeter and Spectrophotometer (Vijay Kumar, Kiran Dip Gill)....Pages 17-20 Preparation of General Laboratory Solutions and Buffers (Vijay Kumar, Kiran Dip Gill)....Pages 21-27 Examination of Urine for Normal Constituents (Vijay Kumar, Kiran Dip Gill)....Pages 29-32 To Perform Qualitative Tests for Urinary Proteins (Vijay Kumar, Kiran Dip Gill)....Pages 33-37 To Determine the Quantity of Proteins in Urine Sample Using Biuret Reaction (Vijay Kumar, Kiran Dip Gill)....Pages 39-41 To Estimate the Amount of Total Protein and Albumin in Serum and to Find A/G Ratio (Vijay Kumar, Kiran Dip Gill)....Pages 43-48 To Perform Qualitative Test for Reducing Substances in Urine (Vijay Kumar, Kiran Dip Gill)....Pages 49-52 Quantitative Analysis of Reducing Sugars in Urine (Vijay Kumar, Kiran Dip Gill)....Pages 53-55 Estimation of Blood Glucose Levels by Glucose Oxidase Method (Vijay Kumar, Kiran Dip Gill)....Pages 57-60 To Determine the Blood Glucose Levels by Folin and Wu Method (Vijay Kumar, Kiran Dip Gill)....Pages 61-62 To Perform Glucose Tolerance Test (Vijay Kumar, Kiran Dip Gill)....Pages 63-66 Estimation of Urea in Serum and Urine (Vijay Kumar, Kiran Dip Gill)....Pages 67-70 To Determine Urea Clearance (Vijay Kumar, Kiran Dip Gill)....Pages 71-73 To Estimate Creatinine Level in Serum and Urine by Jaffe’s Reaction (Vijay Kumar, Kiran Dip Gill)....Pages 75-78 To Determine Creatinine Clearance (Vijay Kumar, Kiran Dip Gill)....Pages 79-80 To Determine the Uric Acid Concentration in Serum and Urine (Vijay Kumar, Kiran Dip Gill)....Pages 81-84 Estimation of Total Calcium in Serum and Urine (Vijay Kumar, Kiran Dip Gill)....Pages 85-88 Estimation of Inorganic Phosphorus in Serum and Urine (Vijay Kumar, Kiran Dip Gill)....Pages 89-92 To Estimate the Amount of Total Cholesterol in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 93-95 To Estimate Total and Direct Bilirubin in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 97-101 To Determine Alanine and Aspartate Transaminase Activity in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 103-106 To Estimate the Activity of Alkaline Phosphatase in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 107-109 To Estimate the Activity of Acid Phosphatase in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 111-112 To Determine Serum and Urinary Amylase Activity (Vijay Kumar, Kiran Dip Gill)....Pages 113-115 To Estimate the Activity of Lipase in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 117-118 Qualitative Analysis of Ketone Bodies in Urine (Vijay Kumar, Kiran Dip Gill)....Pages 119-122 Qualitative Test for Bile Pigments and Urobilinogen in Urine (Vijay Kumar, Kiran Dip Gill)....Pages 123-127 Determination of Total Lactate Dehydrogenase Activity in Serum Sample (Vijay Kumar, Kiran Dip Gill)....Pages 129-130 To Measure Activity of Creatine Kinase in Serum (Vijay Kumar, Kiran Dip Gill)....Pages 131-133 Analysis of Cerebrospinal Fluid for Proteins and Sugars (Vijay Kumar, Kiran Dip Gill)....Pages 135-137 To Measure Lipid Profile in Serum Sample (Vijay Kumar, Kiran Dip Gill)....Pages 139-145 To Estimate Sodium and Potassium in Serum by Using Flame Photometer (Vijay Kumar, Kiran Dip Gill)....Pages 147-150 To Perform Radioimmunoassay (Vijay Kumar, Kiran Dip Gill)....Pages 151-152 To Perform Enzyme-Linked Immunosorbent Assay (Vijay Kumar, Kiran Dip Gill)....Pages 153-155 Some Important Case Studies (Vijay Kumar, Kiran Dip Gill)....Pages 157-167 Back Matter ....Pages 169-175