ENGLISH

Cancer Immunosurveillance: Methods and Protocols

By Alejandro Lopez-Soto; Alicia Folgueras

Book information

Publisher
Humana Press
Year
2018
ISBN
1493988840, 9781493988846
Language
english
Format
PDF
Filesize
11 MB (11571454 bytes)
Edition
Hardcover
Pages
363\365
Time added
2018-11-24 14:00:56

Description

This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough,Cancer Immunosurveillance: Methods and Protocolsis a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field. Front Matter ....Pages i-xiii Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer (Willem H. Zoutman, Rogier J. Nell, Pieter A. van der Velden)....Pages 1-14 An Economical, Quantitative, and Robust Protocol for High-Throughput T Cell Receptor Sequencing from Tumor or Blood (Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton, on behalf of the TRACERx consortium et al.)....Pages 15-42 Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells (Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song)....Pages 43-55 Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Antitumor Effects In Vivo (Akshat Sharma, Nicholas A. Zumwalde, Jenny E. Gumperz)....Pages 57-72 Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors (Yong Yu, Pengtao Liu)....Pages 73-86 Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance (Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale)....Pages 87-117 CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity (Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, Alejandro López-Soto)....Pages 119-130 A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anticancer Immune Responses In Vitro (Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González, Alejandro López-Soto)....Pages 131-139 Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency (Vidhya R. Nair, Srinivas Malladi)....Pages 141-150 Tumor-Associated Macrophage Isolation and In Vivo Analysis of Their Tumor-Promoting Activity (Nicholas M. Clark, Paula D. Bos)....Pages 151-160 Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo (Magali Dupont, Christian A. J. Vosshenrich)....Pages 161-176 Determination and Isolation of Immune Populations from Brain Tumor Microenvironments (Javier Godino, Alberto J. Schuhmacher)....Pages 177-188 Mouse Model of Colitis-Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells (Llipsy Santiago, Marta Castro, Julián Pardo, Maykel Arias)....Pages 189-202 Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry (Shelly Kalaora, Yardena Samuels)....Pages 203-214 Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways (Cherie’ R. Scurrah, Alan J. Simmons, Ken S. Lau)....Pages 215-229 Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation (Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, Jerome Paggetti)....Pages 231-245 Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion (Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, Cristina Raimondi)....Pages 247-258 Quantitative Identification of Senescent Cells in Cancer (Anat Biran, Ziv Porat, Valery Krizhanovsky)....Pages 259-267 Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance (Dirk Zboralski, Anna Frömming)....Pages 269-281 Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment (Xiuyun Jiang, Y. David Seo, Kevin M. Sullivan, Venu G. Pillarisetty)....Pages 283-295 Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models (Juliette Humeau, Sarah Lévesque, Guido Kroemer, Jonathan G. Pol)....Pages 297-315 A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers (Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, Jessica Dal Col)....Pages 317-333 Development of Bispecific Antibody Derivatives for Cancer Immunotherapy (Yuan He, Wijnand Helfrich, Edwin Bremer)....Pages 335-347 Generation of CAR-T Cells for Cancer Immunotherapy (Qumiao Xu, Hizkia Harto, Robert Berahovich, Shirley Xu, Hua Zhou, Vita Golubovskaya et al.)....Pages 349-360 Back Matter ....Pages 361-363

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