ENGLISH

Cell-free DNA as Diagnostic Markers: Methods and Protocols

By Valentina Casadio , Samanta Salvi

Book information

Publisher
Springer New York,Humana Press
Year
2019
ISBN
978-1-4939-8972-0, 978-1-4939-8973-7
Language
english
Format
PDF
Filesize
5 MB (5386860 bytes)
Series
Methods in Molecular Biology 1909
Edition
1st ed.
Pages
X, 224\215
Time added
2019-01-12 07:29:56
Biomedicine; Human Genetics; Cancer Research

Description

This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-x Front Matter ....Pages 1-1 Cell-Free DNA: Applications in Different Diseases (Rossella Ranucci)....Pages 3-12 Cell-Free DNA: An Overview of Sample Types and Isolation Procedures (Filippo Martignano)....Pages 13-27 Front Matter ....Pages 29-29 Circulating Cell-Free DNA and Cancer Therapy Monitoring: Methods and Potential (Peter B. Gahan)....Pages 31-46 Cell-Free DNA in the Liquid Biopsy Context: Role and Differences Between ctDNA and CTC Marker in Cancer Management (Pietro Fici)....Pages 47-73 Front Matter ....Pages 75-75 Cell-Free DNA Integrity: Applications (Sara Ravaioli)....Pages 77-83 Copy Number Variation Analysis on Cell-Free Serum DNA (Heidi Schwarzenbach)....Pages 85-93 Studying Copy Number Variations in Cell-Free DNA: The Example of AR in Prostate Cancer (Samanta Salvi, Valentina Casadio)....Pages 95-103 dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues (Takashi Takeshita, Hirotaka Iwase)....Pages 105-118 From cfDNA to Sequencing: Workflows and Potentials (Michela Tebaldi, Samanta Salvi)....Pages 119-125 Front Matter ....Pages 127-127 Epigenetic Characterization of Cell-Free DNA (Giorgia Gurioli)....Pages 129-135 Quantitative Methylation-Specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell-Free DNA (Luca Sigalotti, Alessia Covre, Francesca Colizzi, Elisabetta Fratta)....Pages 137-162 Front Matter ....Pages 163-163 Cell-Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges (Lucia Tombolan, Angelica Zin, Gianni Bisogno)....Pages 165-175 Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma (Meng Ni, Xianlu Laura Peng, Peiyong Jiang)....Pages 177-180 Front Matter ....Pages 181-181 Circulating Cell-Free DNA in Physical Activities (Leydi Natalia Vittori, Andrea Tarozzi, Pasqualino Maietta Latessa)....Pages 183-197 Front Matter ....Pages 199-199 Urinary Cell-Free DNA: Potential and Applications (Samanta Salvi, Valentina Casadio)....Pages 201-209 Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment (Valentina Casadio, Samanta Salvi)....Pages 211-221 Back Matter ....Pages 223-224

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