ENGLISH

Dengue: Methods and Protocols

By Radhakrishnan Padmanabhan , Subhash G. Vasudevan (eds.)

Book information

Publisher
Humana Press
Year
2014
ISBN
1493903470, 9781493903474
DOI
10.1007/978-1-4939-0348-1
ISSN
1064-3745
LCC
2014933052
Language
english
Format
PDF
Filesize
18 MB (19232640 bytes)
Series
Methods in Molecular Biology 1138
Edition
1
Pages
403\419
Topic
Biology Virology
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2014-04-09 15:30:55
Infectious Diseases; Virology; Laboratory Medicine

Description

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

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