ENGLISH

Directed Evolution: Methods and Protocols (Methods in Molecular Biology, 2461)

By Andrew Currin (editor) , Neil Swainston (editor)

Book information

Publisher
Humana
Year
2022
ISBN
1071621513, 9781071621516
Language
english
Format
PDF
Filesize
7 MB (7377377 bytes)
Pages
289\281
Time added
2024-03-21 08:27:45

Description

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

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