ENGLISH

Organelle Proteomics

By Edein P. Romijn , John R. Yates III (auth.) , Delphine Pflieger , Jean Rossier (eds.)

Book information

Publisher
Humana Press
Year
2008
ISBN
9781588297792, 1588297799
DOI
10.1007/978-1-59745-028-7
LCC
QH581.2 O74 2008
Open Library ID
OL12325733M
Language
english
Format
PDF
Filesize
4 MB (4320521 bytes)
Series
Methods in Molecular Biology 432
Edition
1
Pages
421\424
Topic
Biology
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
generalProteomics; Cell Biology; Biochemistry

Description

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

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