Date palm biotechnology protocols. Volume I, Tissue culture applications

Description

This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications is comprised of 27 chapters covering adventitious organogenesis, somatic embryogenesis, contamination, hyperhydricity, acclimatization, cell suspension, protoplast and bioreactors, genetic transformation secondary metabolites, and abiotic stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology. Read more... Abstract: This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications is comprised of 27 chapters covering adventitious organogenesis, somatic embryogenesis, contamination, hyperhydricity, acclimatization, cell suspension, protoplast and bioreactors, genetic transformation secondary metabolites, and abiotic stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology Front Matter ....Pages i-xiii Front Matter ....Pages 1-1 Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants (Larbi Abahmane)....Pages 3-15 NAA-Induced Direct Organogenesis from Female Immature Inflorescence Explants of Date Palm (Hussam S. M. Khierallah, Saleh M. Bader, Makki A. Al-Khafaji)....Pages 17-25 Direct Organogenesis from Immature Female Inflorescence of Date Palm by Gradual Reduction of 2,4-D Concentration (Ezz El-Din G. Gadalla)....Pages 27-35 Optimized Direct Organogenesis from Shoot Tip Explants of Date Palm (Rehab Sidky)....Pages 37-45 Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State (Eman M. M. Zayed)....Pages 47-59 Front Matter ....Pages 61-61 Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater (Rania A. Taha)....Pages 63-75 Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2,4-D Concentration (Zeinab E. Zayed)....Pages 77-88 Indirect Somatic Embryogenesis from Mature Inflorescence Explants of Date Palm (Ali M. Al-Ali, Chien-Ying Ko, Sultan A. Al-Sulaiman, Sami O. Al-Otaibi, Abd Ulmoneem H. Al-Khamees, Megahed H. Ammar)....Pages 89-97 Indirect Somatic Embryogenesis of Date Palm Using Juvenile Leaf Explants and Low 2,4-D Concentration (Lotfi Fki, Walid Kriaa, Ameni Nasri, Emna Baklouti, Olfa Chkir, Raja B. Masmoudi et al.)....Pages 99-106 Desiccation-Enhanced Maturation and Germination of Date Palm Somatic Embryos Derived from Cell Suspension Culture (Nazim Boufis, Khayreddine Titouh, Lakhdar Khelifi)....Pages 107-117 Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination (Hussein J. Shareef)....Pages 119-128 Histological Evidence of Indirect Somatic Embryogenesis from Immature Female Date Palm Inflorescences (Eman M. M. Zayed, Ola H. Abdelbar)....Pages 129-144 Histological Analysis of the Developmental Stages of Direct Somatic Embryogenesis Induced from In Vitro Leaf Explants of Date Palm (Ola H. Abdelbar)....Pages 145-162 Front Matter ....Pages 163-163 Identifying and Controlling Contamination of Date Palm Tissue Cultures (Abeer H. I. Abdel-Karim)....Pages 165-174 Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients (Maiada M. El-Dawayati, Zeinab E. Zayed)....Pages 175-183 Improvement of In Vitro Date Palm Plantlet Acclimatization Rate with Kinetin and Hoagland Solution (Mona M. Hassan)....Pages 185-200 Front Matter ....Pages 201-201 Plant Regeneration from Somatic Embryogenic Suspension Cultures of Date Palm (Mansour A. Abohatem, Yazid Bakil, Mohmmed Baaziz)....Pages 203-214 Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid (Hussain A. Alwael, Poornananda M. Naik, Jameel M. Al-Khayri)....Pages 215-226 Microcalli Induction in Protoplasts Isolated from Embryogenic Callus of Date Palm (Khayreddine Titouh, Nazim Boufis, Lakhdar Khelifi)....Pages 227-237 Temporary Immersion System for Date Palm Micropropagation (Ahmed Othmani, Chokri Bayoudh, Amel Sellemi, Noureddine Drira)....Pages 239-249 Plantform Bioreactor for Mass Micropropagation of Date Palm (Abdulminam H. A. Almusawi, Abdullah J. Sayegh, Ansam M. S. Alshanaw, John L. Griffis Jr)....Pages 251-265 Front Matter ....Pages 267-267 Genetic Transformation of Date Palm Via Microprojectile Bombardment (Mousa Mousavi, Amir Mousavi, Ali A. Habashi, Kazem Arzani, Bahareh Dehsara, Mohsen Brajeh)....Pages 269-280 Microprojectile Bombardment Transformation of Date Palm Using the Insecticidal Cholesterol Oxidase (ChoA) Gene (Mai A. Allam, Mahmoud M. Saker)....Pages 281-293 Transient GUS Gene Expression in Date Palm Fruit Using Agroinjection Transformation Technique (Mohei El-Din M. Solliman, Hebaallah A. Mohasseb, Abdullatif A. Al-Khateeb, Jameel M. Al-Khayri, Suliman A. Al-Khateeb)....Pages 295-305 Front Matter ....Pages 307-307 Bioreactor Steroid Production and Analysis of Date Palm Embryogenic Callus (Sherif El-Sharabasy, Maiada El-Dawayati)....Pages 309-318 Extraction and Estimation of Secondary Metabolites from Date Palm Cell Suspension Cultures (Poornananda M. Naik, Jameel M. Al-Khayri)....Pages 319-332 In Vitro Assessment of Abiotic Stress in Date Palm: Salinity and Drought (Jameel M. Al-Khayri, Poornananda M. Naik, Hussain A. Alwael)....Pages 333-346 Back Matter ....Pages 347-353