Plant Membrane Proteomics: Methods and Protocols

Description

This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field. Front Matter ....Pages i-xii Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis (Bronwyn J. Barkla)....Pages 1-12 Isolation and Purity Assessment of Membranes from Norway Spruce (Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt et al.)....Pages 13-39 Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis (Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty)....Pages 41-55 Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis (Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána et al.)....Pages 57-79 Isolation, Purity Assessment, and Proteomic Analysis of Nuclei (Setsuko Komatsu)....Pages 81-90 Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient (Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko et al.)....Pages 91-105 Analyzing the Vacuolar Membrane (Tonoplast) Proteome (Miwa Ohnishi, Katsuhisa Yoshida, Tetsuro Mimura)....Pages 107-116 Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies (Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphné Seigneurin-Berny, Norbert Rolland)....Pages 117-136 Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria (Ann Magnuson, Tanai Cardona)....Pages 137-145 Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development (Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas et al.)....Pages 147-162 Sample Preparation for Analysis of the Plant Mitochondrial Membrane Proteome (Christine Schikowsky, Beate Thal, Hans-Peter Braun, Holger Eubel)....Pages 163-183 Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS (Bin Li, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura)....Pages 185-194 Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis (Anna M. Jozefowicz, Andrea Matros, Katja Witzel, Hans-Peter Mock)....Pages 195-204 Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses (Małgorzata Janicka, Anna Wdowikowska, Grażyna Kłobus)....Pages 205-215 Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters (Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood)....Pages 217-234 MSE for Label-Free Absolute Protein Quantification in Complex Proteomes (Stefan Helm, Sacha Baginsky)....Pages 235-247 Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON (Rainer Schwacke, Ulf-Ingo Flügge)....Pages 249-259 VANTED: A Tool for Integrative Visualization and Analysis of -Omics Data (Anja Hartmann, Anna Maria Jozefowicz)....Pages 261-278 Back Matter ....Pages 279-280